摘要
目的:调查市售红参须质量,制定红参须的质量标准。方法:收集不同地区市售红参须样品,采用性状观察,显微、薄层色谱鉴别,总灰分检查,高效液相色谱进行含量测定。结果:10批样品外观性状有差异;显微特征明显;薄层色谱中在与人参对照药材、人参皂苷Rb1、人参皂苷Re、人参皂苷Rf及人参皂苷Rg1相应的位置上显相同颜色的斑点;总灰分为2.50%～4.26%;人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1的线性范围分别为0.117～1.750,0.207～3.100,0.298～4.470μg,回收率分别为94.4%,95.2%,95.7%,RSD分别为1.99%,1.55%,1.54%,含量人参皂苷Rg1+人参皂苷Re为0.228%～0.666%,人参皂苷Rb1为0.312%～0.783%。结论:市售红参须质量差异较大,亟待制定红参须的质量标准。
Objective： To establish the quality standards of Panacis Ginseng Radix Laterlis.Method： This experiment collected the Panacis Ginseng Radix Laterlis from different areas,which is to observe the characters,identify the quality by microscopy and TLC,examine the total ash,make the content determination by HPLC.Result： The appearance of 10 batches of samples was different when micro-characteristic were clear.The TLC spots of samples have the same color with Ginseng Radix et Rhizoma,ginsenoside Rb1,Re,Rf,Rg1.however,the total ash was 2.50%-4.26%.ginsenoside Rg1,Re,Rb1 has a good linearity within 0.117-1.750 μg,0.207-3.100 μg,0.298-4.470 μg and the average recovery was 94.4%,95.2%,95.7% with RSD 1.99%,1.55%,1.54%.The content of ginsenoside Rg1 ＋ Re in 10 batchs of samples is 0.228%-0.666%,and the content of ginsenoside Rb1 is 0.312%-0.783%.Conclusion： The quality of Panacis Ginseng Radix Laterlis from different areas is considerably different to be established urgently.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第14期124-127,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
2012年版<山东省中药材标准>研究项目
作者简介
[通讯作者]刘善新，硕士，副研究员，从事中药新制剂、新剂型研究，Tel：0531-82949812，E-mail：liushanxin66@163．com
