摘要
采用逐渐改变盐度的方法,结合相关免疫酶学测定方法,研究盐度由34渐变至16、22、28、40后,48 h内杂交鲍〔皱纹盘鲍(Haliotis discus hannai Ino,♀)×日本盘鲍(H.discus discus Ino,♂)〕血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、碱性磷酸酶(AKP)、溶菌酶(LZM)、抗菌活力(Ua)的变化规律,旨在研究盐度渐变对杂交鲍免疫相关因子的影响。结果显示,盐度28、34(对照)组5种免疫因子变化均不显著。盐度40组,AKP活力极显著低于对照组;CAT活力显著升高,而后逐渐降低,最终显著低于对照组;LZM活力下降后逐渐稳定在一个显著低于对照组的值。盐度22组,AKP活力有下降的趋势,在48 h时显著低于对照组。盐度16组SOD、AKP、LZM、Ua均有下降的趋势,并且在48 h时均显著低于对照组;整个试验过程中,CAT活力均显著低于对照组。结果表明,盐度接近或高于40及接近或低于16将显著影响杂交鲍5种免疫因子的活力,进而影响其免疫力。
Hybrid abalones [Haliotis discus hannai Ino（ ♀ ）×H. discus discus Ino（ ♂ ））, were exposed to different salinity in their all life, but how immune parameters changeed when the salinity changed was still uncertain. In this study, we investigated the changes of serum superoxide dismutase （SOD）, alkaline phosphatase （AKP）, catalase （CAT）, lysozyme （LZM） and bactericidal （Ua） activities when the salinity stepwise changed from 34 to 16, 22, 28, 40 within 48 h. It aimed to study effects of gradient salinity on immune-related factors. The results showed that five immune factors change was not significant at salinity 28, 34 （control group） during the experiment. In salinity 40 group, AKP activity was significantly lower than the control group in all experiment, CAT activity significantly increased at first and then gradually decreased, significantly lower than the control group at last level, LZM activity declined and gradually stayed stable at a significantly lower value than the control group at last. In salinity 22 group, AKP activity had a downward trendy, and significantly lower than the control group at 48 h. In salinity 16 group, SOD, AKP, LZM activity and bactericidal activities had a downward trend, significantly lower than the control group at 48 h, CAT vitality was significantly lower than the control group in all detection periods. The results indicated that when the salinity closed to or higher than 40 and closed to or below 16, the activity of serum immune factors even immunity in hybrid abalone were significantly influenced.
出处
《广东农业科学》
CAS
CSCD
北大核心
2013年第12期136-139,共4页
Guangdong Agricultural Sciences
基金
国家现代农业产业技术体系建设专项(CARS-48)
广东省农业攻关项目(2010B20201014)
关键词
盐度
杂交鲍
免疫因子
抗菌活力
salinity
Haliotis discus hannai Ino
immune factors
bactericidal activities
作者简介
作者简介：时少坤（1987-），男，在读硕士生，E-mail：shaokun1717@163．com
通讯作者：王江勇（1971-），男，博士，研究员，E-raMl：wjy104@163．com
