摘要
为了对新疆布鲁氏菌分离株80/23株进行分型鉴定,本实验采用MLVA-16检测方法和常规生物学鉴定方法对布鲁氏80/23株进行研究,将测定结果与http://mlva.upsud.fr/MLVA net提供的530株布鲁氏菌数据库比较分析,应用UPGMA进行遗传进化树分析,并构建系统进化树。结果显示,常规分型方法鉴定为羊种3型,MLVA方法鉴定该菌株与德国分离株Bruce0261菌株的亲缘关系最近,属为东地中海3型,羊种1型,两种分型方法种属结果一致。结论:MLVA-16与常规生物学鉴定方法相比,具有更高的精确性,对布鲁氏菌生物型和株间差异有很高的鉴别力。并能为布病疫情流行株的溯源进化关系提供依据。
To analyze genotype of Brucella 80/23 strain isolated in Xinjiang, multilocus variable-number tandem-repeat analysis （MLVA-16） and conventional biological identification method were used in this study. Comparative analysis was conducted between our results and Brucella 530 from database （http：//mlva. upsud.fr/MLVA）,and further phylogenetic tree was constructed by using UPGMA. The results showed that the Brucella 80/23 strain was identified as B. melitensis serotype 3 strains by the conventional identity method,and MLVA method displayed Brucella 80/23 strain was close to Bruce0261,belonging to Eastern Mediterranean type,B, melitensis serotype 1 strains. There is the same species between two identity methods. The conclusion was that, compared with the conventional biological identification method, MLVA-16 has higher accuracy for the type and strain of Brucella biological differences. MLVA-16 can provide the basis for traceability and evolutionary relationship of the brucellosis epidemic strain.
出处
《石河子大学学报（自然科学版）》
CAS
2013年第3期313-317,共5页
Journal of Shihezi University(Natural Science)
基金
国家重点基础研究发展计划(973计划)项目(2010CB530203)
国家自然科学基金项目(31060334)
新疆兵团博士基金项目(2009JC15)
作者简介
任晓莉（1987-），女，硕士研究生，研究方向为畜禽病原分子生学，e—mail：lovexiaoli1987@126．com
通信作者：陈创夫（1962-），男，教授，博士生导师，从事分子病理学与免疫遗传研究，e-mail：ccf-xb@163．com
