摘要
floR是氟苯尼考特异性耐药基因之一,当前宠物犬源大肠杆菌氟苯尼考耐药基因floR的研究报道很少,故本试验利用PCR方法对宠物犬源大肠杆菌氟苯尼考耐药基因floR进行检测,并采用微量肉汤稀释法测定宠物犬源大肠杆菌对10种抗菌药物的最小抑菌浓度(MIC)。结果显示,20株宠物犬源大肠杆菌中floR耐药基因的阳性菌株数为9株,阳性检出率为45%;对氟苯尼考的耐药率为65%,并呈现3～7重的多重耐药性。结果表明,随着氟苯尼考在兽医临床的广泛应用,其耐药率越来越高,需不断加强对氟苯尼考等抗菌药物的耐药性监控。
floR was one of the main specific drug resistance genes to florfenicol. There was a paucity of data for the detection of floR in Escherichia coli isolated from pet dogs. The survey of floR gene by PCR and MICs of 10 common antibiotics by mi- cro-broth dilution were performed in this study. The results indicated that among 20 Escherichia coli isolates, the resistant rate to florfenicol was 65% and there were 9 floR-positive strains, positive rate was 45%. It could be concluded that the resistance rate of florfenicol for pet Escherichia coli isolates would appear animals clinical diseases. So it was very necessary to supervise dogs. continually increasing along with this antibiotic extensive use in florfenicol resistance among Escherichia coli isolates from pet
出处
《中国畜牧兽医》
CAS
北大核心
2013年第6期211-215,共5页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(31072170)
关键词
宠物犬
大肠杆菌
氟苯尼考
floR
多重耐药
pet dogs
Escherichia coli
florfenicol
floR
multiple drug resistance
作者简介
贺志沛（1987-），女，河南人，硕士生，研究方向：兽医药理学及细菌耐药性。
通信作者：吴华（1974-），女，河南人，博士。E—mail：huatong—zhi66@163．com
