摘要
[目的]观察不同组方祛痹镇痛凝胶贴膏穴位敷贴对胶原诱导性关节炎(CIA)大鼠的痛阈及血清前列腺E(2PGE2)的影响。[方法]将SD大鼠随机分成空白对照组和造模组。造模组由牛Ⅱ型胶原诱发CIA模型,造模15天后将大鼠随机分为A、B及C贴膏组、模型对照组、基质组和阳性对照组,各治疗处理组敷贴"身柱"、"至阳"及"命门"三穴,从造模后16天开始,连续治疗15天。以热辐射甩尾法检测大鼠不同时期的痛阈,放射免疫法检测大鼠治疗前后血清中PGE2的含量。[结果]与模型对照组相比,治疗5天后,C贴膏组痛阈明显升高(P<0.05);治疗10天与治疗15天后,不同组方贴膏组和阳性对照组痛阈均有显著的升高(P<0.01,P<0.05)。治疗15天后,与模型对照组相比,B贴膏组血清中PGE2含量有明显升高(P<0.05)。计算各组最大可能作用的百分数(%MPE),A贴膏组%MPE上升了0.37,模型对照组、基质组、阳性对照组、B贴膏组、C贴膏组分别下降12.18、9.22、0.82、7.49与1.15。[结论]不同组方的祛痹镇痛凝胶膏对CIA大鼠均有良好的镇痛作用,其中C贴膏镇痛作用发挥最早;A、C贴膏对血清中PGE2的含量下降有较好的抑制作用。
[Objective]To observe the effects of acupoint application with different formulas of Gel plaster on rats with collagen-induced arthritis（CIA） on the pain threshold and prostaglandin E2（PGE2）.[Methods] The SD rats were randomly divided into blank group and model group.Bovine typeⅡcollagen was injected intradermally to induce CIA model with the model group.After 15 days,the models were divided randomly into a Gel plaste A group,a Gel plaste B group,a Gel plaste C group,a model control group,a matrix control group and a positive control group.The treatment group applied the plaster on Shenzhu（DU 12）,Zhiyang（DU 9） and Mingmen（DU 4） points by continuing treatment of 15 days after modeling 16 days.Using rat radiant heat tail flick test to detect pain threshold in different periods and radio-immunity method to detect serum PGE2 content in different time points.[Results] Compared with model control group,the pain threshold of the Gel plaste C group increased significantly after 5 days treatment（P＆lt;0.05）;after 10 days and 15 days treatment the pain threshold of different formulas of Gel Plaster group increased markedly（P＆lt;0.01,P＆lt;0.05）.Compared with model control group,the content of serum PGE2 of Mateng Gel plaste3 group increased significantly after 15 days treatment（P＆lt;0.05）.Calculating the percentage of maximum possible effect（% MPE）,the Gel plaste A group rose to 0.37,model control group,matrix control group,positive control group,the Gel plastes B group and the Gel plaste C group decreased 12.18,9.22,0.82,7.49 and 1.15 respectively.[Conclusion]Acupoint application with different formulas of Gel Plasters had certain analgesic effect on CIA rats.Among them the Gel plaste C group expressed the analgesic function first;The Gel plaste A group and the Gel plaste C group and positive control group played inbibitional effect on the decline of serum PGE2 content.
出处
《浙江中医药大学学报》
CAS
2013年第5期575-578,共4页
Journal of Zhejiang Chinese Medical University
基金
浙江省科技厅重大科技专项和优先主题项目计划(2010C13014)
浙江省自然科学基金资助项目(Y2111194)
浙江省"重中之重"学科(针灸推拿学)建设经费资助(浙教高科[2008]255号)~~
