摘要
目的探讨乌司他丁对SD大鼠脓毒症脑损伤的保护作用及其机制。方法将SD大鼠随机分为4组：假手术组（Sham）、模型组（cLP）、乌司他丁低剂量组（5x10^4U／kg）和乌司他丁高剂量组（10x10^4U／kg）。采用盲肠结扎穿孔（CLP）法复制脓毒症模型。术后治疗组动物给予乌司他丁5xl04U／kg或10x10^4／kg腹腔注射，1次／日。造模72h后取脑，观察脑组织病理改变及细胞凋亡，并测定各组脑组织一氧化氮（No）、诱导型一氧化氮合酶（iNOS）、一氧化氮合酶（Nos）、活性氧簇（ROS）、丙二醛（MDA）含量及超氧化物歧化酶（SOD）活性变化。结果与Sham组比较，CLP组正常神经元减少，凋亡细胞增加，NO、iNOS、NOS、ROS和MDA含量增加，SOD活性下降（P〈0.01）；与CLP组比较，乌司他丁高、低剂量组以上指标均有所改善（P〈0．05或0．01），且高剂量组较低剂量组改善更明显（P〈0．05）。结论乌司他丁对脓毒症诱发的脑损伤有保护作用，其机制可能与抑制氧／氮自由基生成、抗凋亡有关。
Objective To explore the mechanism of protective effects of ulinastatin on sepsis-induced brain injury in rats. Methods 48 male SD rats were randomly divided into 4 groups （n =12）, Sham group, CLP group, low-dose ulinastatin group （UTI 1）, and high-dose ulinastatin group （UTI 2）. Cecal ligation and puncture （CLP） were used to reproduce septic shock model. Ulinastatin groups of 5x 10^4 U/kg or 10x10^4 U/kg ulinastatin were given via intraperitoneal injection once a day for 3 days after operation, respectively. In sham group, operation was performed without CLP. HE staining and TUNEL method were used to investigate pathologic change and apoptosis at 72-hour after the operation. The NO, iNOS, NOS, MDA contents and the activity of SOD in brain were also investigated. Results Comparing with sham group, it reflected a decreased normal neurons, increased neural rate of cell apoptosis, and higher content of NO, iNOS, NOS, ROS and MDA, with a lower SOD activity in CLP group （P 〈0.01）. Comparing with CLP group, both ulinastatin groups demonstrated an improved parameters （P 〈0.05 or 0.01）. As a matter of fact, the high-dose ulinastatin group reflected an even better improvement than the low-dose ulinastatin group （P 〈0.05）. Conclusion Ulinastatin is considered to provide protection against sepsis-mediated brain injure by decreasing oxygen/nitrogen free radicals and neural cell apoptosis.
出处
《中国急救复苏与灾害医学杂志》
2013年第6期530-532,568,共4页
China Journal of Emergency Resuscitation and Disaster Medicine
关键词
乌司他丁
脓毒症
凋亡
自由基
Ulinastatin
Sepsis
Apoptosis
Free radical
作者简介
通讯作者：廖晓星，Email：liawens@163.com
