摘要
目的研究自体骨髓基质细胞(bone marrow stromal cells,BMSCs)体外诱导扩增与仿生纳米壳聚糖-胶原复合支架材料(nano chitosan sodium collagen scaffold,NCSCS)复合修复骨缺损的可行性。方法分离纯化Wistar大鼠BMSCs,诱导BM-SCs向成骨细胞(osteoblast,OB)转化,经碱性磷酸酶、茜素红组织化学染色鉴定,将BMSCs复合仿生纳米壳聚糖-胶原复合支架材料(nano chitosan sodium collagen scaffold,NCSCS)进行扫描电镜观察。制作Wistar大鼠胫骨骨缺损模型,缺损处NCSCS-BMSCs复合物移植后观察骨缺损修复成骨情况。结果扫描电镜下BMSCs细胞在仿生纳米壳聚糖-胶原复合支架材料上大面积生长。BMSCs复合仿生纳米壳聚糖-胶原复合支架材料植入2周后,材料周围可见大量纤维组织,并可见成骨细胞和少量新生的骨样基质,新生骨对照组和实验组比较差异显著(P<0.01);植入6周后,可见到材料与新生骨之间相互混杂,中有纤维相间隔,和原有骨界面尚清晰可辨认,材料部分降解,对照组和实验组比较新生骨有统计学差异(P<0.05);植入12周后,材料几乎降解完全,和天然骨组织界面可见新骨形成,对照组和实验组比较新骨形成无统计学差异(P>0.05)。材料降解在各时间段对照组和实验组比较差别不显著(P>0.05)。结论 BMSCs介导NCSCS修复骨缺损优于单纯的NCSCS修复骨缺损,尤以早期效果为好。
Objective To study the feasibility of bone defect repair with in vitro induced BMSCs combined with NCSCS material.Methods Isolate and purify Wistar rat BMSCs,and induce BMSCs to osteoblasts,which were identified by alkaline phosphataseand and alizarin red histochemical staining.BMSCs on the NCSCS were observed by scanning electron microscope.Wistar rat tibia bone defect model was built.Implant NCSCS-BMSCs complex material to bone defects,and observe the bone formation.Results Large scale of BMSCs proliferated on the NCSCS material under scanning electron microscope.Two weeks after the NCSCS-BMSCs were implanted,a great amount of fiber tissues with osteoblasts and some osteoid matrix were found around the material.There were statistical differencein the percentage of new bone between the experimental and the control groups（P〈0.01）.6 weeks after NCSCS-BMSCs were implanted,new bone infiltrated into NCSCS,with fiber between them,and the original bone interface was still clear and could still be identified.Part of the NCSCS were degraded,and there was statistical difference in the percentage of new bone between the experimental and the control groups（P〈0.05）.12 weeks after the NCSCS-BMSCs were implanted,NCSCS were almost entirely degraded,and new bone was found on the original bone interface.There was no difference in new bone formation between these two groups（P〈0.05）.The degradation of the NCSCS showed no significant difference between the experimental and the control groups at different time points.Conclusions NCSCS-BMSCs are better than NCSCS alone in repair of bone defects,especially in the early-term effect.
出处
《口腔医学》
CAS
2013年第6期371-375,共5页
Stomatology
基金
2009年度余杭区科技发展计划项目(15)
作者简介
通信作者：王芳Tel：（0）18968061573E—mail：wf7175@163．com
