摘要
目的:观察丹皮酚(Pae)对脂多糖(LPS)诱导的大鼠单核细胞(MC)与血管内皮细胞(VEC)黏附功能的影响,研究Pae抗动脉粥样硬化作用的炎症机制。方法:组织块预消化贴壁法分离培养大鼠血管内皮细胞,采用LPS诱导VEC的损伤;健康大鼠灌胃Pae制备含药血清,RP-HPLC法测定血清中Pae含量;MTT法检测Pae对VEC的保护作用;孟加拉玫瑰红染色法检测Pae对LPS诱导的MC与VEC黏附的影响。结果:筛选LPS诱导大鼠MC与VEC黏附的最适浓度为10 ng/mL,作用时间为5 h;以Pae浓度为1.25、2.5、5、10、20μg/mL对损伤的VEC进行干预,当Pae(2.5、5、10μg/mL)作用24 h可有效抑制LPS诱导的MC与VEC的黏附;显著提高LPS导致的VEC存活率的下降。结论:LPS对VEC具有损伤作用,Pae通过提高VEC的存活率,抑制MC与VEC的黏附,以保护VEC免受LPS的损伤。
Objective：To observe the effect of Paeonol（Pae） on lipopolysaccharide（LPS） induced rat mononuclear cells（MCs） adhesion to vascular endothelial cells（VECs） and provide basis foundation for inflammatary mechanisms of Pae against atherosclerosis.Methods：Rat vascular endothelial cells were isolated with tissue predigested adherent method.LPS was used as stimulator to induce VEC injury.Serum containing Pae obtained from healthy rats which were given Pae in intragastric.RP-HPLC method was used for detecting the concentration of Pae in serum.MTT assay was used to determine the protective effect of Pae on injured VECs.Rose Bengal Staining was used to detect the effect of Pae on LPS-induced MCs adhesion to VECs.Results：LPS induced rat MCs adhesion to VECs.The effect was the strongest when the concentration was 10 ng/mL and incubated with VECs for 5 h.Pae in concentration of 2.5,5 and 10 μg/mL and incubated for 24 h could effectively inhibit the adhesion and improve the survival rate of LPS injured VECs significantly.Conclusion：LPS can damage VECs.Pae could protect VECs from LPS injury via inhibiting MCs adhesion to VECs and improving the VEC survival rate.
出处
《中药材》
CAS
CSCD
北大核心
2013年第3期433-437,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(81073090
81274134)
关键词
丹皮酚
血管内皮细胞
脂多糖
黏附
Paeonol
Vascular endothelial cell
Lipopolysaccharide
Adhesion
作者简介
陈君君（1988-），女，在读硕士研究生，专业方向：中药药理学：E—mail：chenjunjun8812@sina．com。
通讯作者：戴敏，Tel：0551-5169221，Fax：0551-5169222。E-mail：daiminliao@163．com。
