摘要
研究四川猪肉产业链中沙门氏菌的污染和传播情况。自2010年5月至2011年5月从四川部分地区的大型养猪场、下游生猪屠宰场和肉类市场采样829份,采用沙门氏菌科玛嘉显色培养基等分离筛选疑似沙门氏菌,并应用针对沙门氏菌invA和hut基因的二重PCR方法鉴定,再对沙门氏菌分离株进行血清型分析。结果表明:分离鉴定出沙门氏菌共112株;养殖场生猪、屠宰场猪肉和销售市场猪肉中沙门氏菌污染率分别为16.40%、10.71%和10.00%;所检出沙门氏菌共有11种血清型,以德尔卑(S.derby)、鼠伤寒(S.typhimurium)、波茨坦(S.potsdam)、吉韦(S.give)等沙门氏菌为主,优势血清型沙门氏菌呈现由养猪场向生猪屠宰场和肉类市场传播的趋势,需要加强卫生监督。
To investigate the contamination and propagation status of Salmonella in pork industry chains from Sichuan province, and to provide a basis for food safety. CHROMagar was used for isolating Salmonella from 829 samples collected from large-scale pig farms, downstream slaughterhouses and sale markets in Sichuan during May 2010 to May 2011 .These strains were identified by duplex PCR amplification of the invA and hut genes. Finally, serotyping of these Salmonella isolates was performed. The results showed that 112 strains of Salmonella were identified from the above samples. Of the samples from pig farms, slaughterhouses and sale markets, 16.40%, 10.71% and 10.00% were found to be positive for Salmonella, respectively. A total of 11 Salmonella serotypes were found, and the dominant serotypes were S. derby, S. （yphimurium, S. potsdam and S. give. These dominant serotypes tended to spread from slaugherhouses to sale markets. As a consequence, the hygine supervision needs to be strengthened.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第11期250-253,共4页
Food Science
基金
国家公益性行业(农业)科研专项(200903055)
关键词
沙门氏菌
猪肉产业链
分离鉴定
血清型
Salmonella
pork industry chain
isolation and identification
serotype
作者简介
侯小刚（1986-），男，硕士研究生，研究方向为食品微生物。E—mail：houxg218@126．com
通信作者：刘书亮（1968-），男，教授，博士，研究方向为食品微生物与发酵工程。E—mail：lsliang999@163．com
