摘要
使用中子活化分析方法测定了富铬酵母与普通酵母的ＤＮＡ、ＲＮＡ和蛋白质中铬的含量，研究铬与ＤＮＡ、ＲＮＡ和蛋白质的结合情况。结果发现，两种酵母的ＤＮＡ、ＲＮＡ和蛋白质提取率和浓度无明显差异，但富铬酵母中ＤＮＡ、ＲＮＡ和蛋白质的铬含量高于普通酵母，而且铬与ＤＮＡ的结合量明显高于ＲＮＡ和蛋白质，说明酵母细胞在含铬的培养基中培养时，铬进入细胞体内产生生物转化，并与酵母细胞的ＤＮＡ、ＲＮＡ和蛋白质发生了结合。
The contents of chromium in the DNA, RNA and protein fractions separated from chromium-rich and normal brewer's yeast were determined with the neutron activation analysis in order to study the combination of Cr with DNA, RNA and protein in chromium-rich brewer's yeast. Our results showed that the extracting rats and concentrations of DNA, RNA and protein had no significant difference in two types of yeast, but the chromium contents of DNA, RNA and protein in the chromium-rich yeast were significantly higher than those in the normal. In addition, the content of chromium in DNA was much higher than that in RNA and protein, which indicated that the inorganic chromium compounds entered into the yeast cell, during the yeast cultivation in the culture medium containing chromium were converted into organic chromium compounds combined with DNA, RNA and protein.
出处
《核技术》
CAS
CSCD
北大核心
2000年第4期231-235,共5页
Nuclear Techniques
基金
国家自然科学基金"八五"重大项目!19392100
中国科学院重大项目!21039751
关键词
铬
富铬酵母
DNA
RNA
蛋白质
中子活化分析
Chromium, Chromium-rich brewer's yeast, DNA, RNA, Protein, Neutron activation analysis
