摘要
目前对徂徕林蛙的描述仅限形态学分析,对其与近缘物种的分化及物种有效性尚缺乏分子生物学研究。笔者对徂徕林蛙所属长肢林蛙种组物种及其他林蛙属物种的线粒体16SrRNA基因部分序列进行分析,得到序列长度521bp。遗传变异分析显示徂徕林蛙与镇海林蛙以及峨眉林蛙遗传距离很近(均为0.9%),表明徂徕林蛙在进化过程中是一个紧密连接镇海林蛙与峨眉林蛙的物种。系统发育树分析显示徂徕林蛙与镇海林蛙构成姐妹群,徂徕林蛙与镇海林蛙处于物种分化过程中的并系阶段,其物种分化还未完全完成。
Rana culaiensis found in Mt.Culai in Shandong,China was reported as a new species just based on morphological analysis;there are no molecular data about the phylogenetic relationships and speciation process for this frog.The partial sequences（521 bp） of 16S rRNA gene from 25 specimens of the brown frog species distributed in China and Japan were analyzed.The nucleotide sequence divergence between R.culaiensis and R.zhenhaiensis was 0.9%,as well as between R.culaiensis and R.omeimontis.The phylogenetic relationships based on NJ and ML indicated that R.culaiensis and R.zhenhaiensis constitute a sister group.It is suggested that R.culaiensis was closely related to R.zhenhaiensis,and the speciation of R.culaiensis are in paraphyletic stage and not completed.
出处
《沈阳师范大学学报（自然科学版）》
CAS
2013年第2期143-147,共5页
Journal of Shenyang Normal University:Natural Science Edition
基金
国家林业局全国第二次陆生野生动物资源调查项目
沈阳师范大学第十批校级重点课程建设项目
沈阳师范大学实验中心主任基金资助项目(SY200902)
作者简介
杨宝田（1963-），男，辽宁沈阳人，沈阳师范大学副教授，博士，硕士研究生导师。
