摘要
以甜瓜抗病资源PI511890和感病自交系‘白皮脆’及其F1、BC1P1、BC1P2、F2群体为材料,苗期接种蔓枯病菌(Didymella bryoniae)进行遗传分析。结果表明:(1)对甜瓜苗期蔓枯病菌接种鉴定结果显示,抗源PI511890的抗病基因Gsb-3由显性单基因控制。(2)利用集团分离分析及ISSR分析技术,引物ISSR-100扩增出的多态性条带与Gsb-3表现连锁关系,该多态性片段大小为900bp。(3)统计ISSR-100在182个F2单株上的多态性,并用Join-Map 4.0软件分析结果显示,ISSR-100与Gsb-3遗传连锁距离为8.3cM,定名为ISSR-100900。研究认为,ISSR-100900可作为甜瓜抗蔓枯病分子育种的备选分子标记。
Segregating population consisting of F1,BC1P1,BC1P2 and F2 were generated by crossing the resistant inbred line PI511890 with the susceptible inbred line＇Baipicui＇.The resistance of segregating population and parental lines were evaluated by inoculating spore isolates of gummy stem blight at seeding stage.The results indicated that：（1）The resistance to gummy stem blight in PI511890 is controlled by a single dominant gene.（2）Using bulked segregation analysis,an unique 900 bp fragment was amplified in resistant materials with the primer ISSR-100.It was confirmed that the marker ISSR-100900 was linked to the gene Gsb-3.（3）The polymorphic patterns of ISSR-100 in the 182 individuals of F2 were scored and then calculated by the software of JoinMap 4.0.The results showed that the genetic distance between ISSR-100 and Gsb-3 is 8.3 cM.This ISSR band indicated that the marker could be useful for marker assisted selection in melon breeding for gummy stem blight resistance.
出处
《西北植物学报》
CAS
CSCD
北大核心
2013年第2期261-265,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
新疆自治区高技术研究与发展项目(201111119)
国家西甜瓜产业技术体系项目(CARS-26-04)
关键词
甜瓜
蔓枯病
抗病基因
ISSR分子标记
Cucumis melo L.
gummy stem blight
disease resistance gene
ISSR marker
作者简介
张学军（1980-），男，硕士，助理研究员，主要从事甜瓜西瓜抗病育种研究。Email：zxj333@126．com
通信作者：伊鸿平，研究员，主要从事甜瓜西瓜遗传育种研究。Email：yhp2387@yahoo．com．cn
