摘要
目的:研究真核细胞翻译起始因子4E结合蛋白(4EBP1)及核糖体40S小亚基S6蛋白激酶(P70S6K)在不同宫颈病变组织中的表达水平及其在宫颈癌发生、发展中的作用。方法:采用免疫组化SP法及PT-PCR法检测宫颈癌、宫颈上皮内瘤变(CIN)及正常宫颈组织中的4EBP1和P70S6K表达水平。结果:在正常宫颈、CIN及宫颈癌组织中,4EBP1和P70S6K蛋白及mRNA水平表达依次增高,且3组比较差异有统计学意义(P<0.05)。4EBP1和P70S6K在宫颈癌中的表达水平呈正相关(r=0.354,P<0.05)。结论:4EBP1和P70S6K在宫颈癌发生、发展中起促进作用,二者可作为宫颈癌诊断及靶向治疗的重要生物学标记物。
Objective： To evaluate the expression of 4EBP1 and P70S6K in different cervical lesions and the functions of 4EBP1 and PTOS6K in the progression of cervical cancer. Methods： The expressions of 4EBP1 and PTOS6K in normal cervix uteri, cervical intraepithelial neoplasia and cervical cancer were detected by the immunohistochemistry （SP methods） and RT - PCR. Results： In normal cervix uteri, cervical intraepithelial neoplasia and cervical carcinomas, both mRNA and protein of 4EBP1 and PTOS6K were successively increased （ P 〈 0.05 ）. There was a positive correlation between 4EBP1 and P70S6K expression in cervical cancer （ r = 0. 354, P 〈 0. 05 ） . Conclusion：High expressions of 4EBPI and PTOS6K may play a promoting role in the pathogenesis and development of cervical cancer. Both of them can be used as important biological markers in diagnosis and targeted therapy of cervical cancer.
出处
《中国妇幼保健》
CAS
北大核心
2013年第12期1959-1962,共4页
Maternal and Child Health Care of China
基金
河南省基础与前沿技术研究计划项目经费支持〔122300410043〕
作者简介
通讯作者E—mail：wangluwen_688@sohu．com
