摘要
以氯仿为除菌介质 ,在温度为 2 8℃ ,pH值为 7.5及搅拌直径比为 2 / 3条件下 ,从发酵液中提取透明质酸 (HA) ,其提取率大于 97.5% ,纯度大于 97.3% .
A new process which removes cells from fermentation suspension by chloroform had been developed. The optimal conditions were 28 ℃、 pH 7.5 and 2/3 ratio of stirrer diameter to stirred bank diameter. Under such conditions, a hyaluronic acid purity of 97.37% and a extraction rate of 97.5% were obtained.
