摘要
为了研究噬菌体尾丝蛋白gp35、gp38在噬菌体宿主识别中的作用,扩增并克隆gp35、gp38基因,分别构建重组表达质粒,得到重组融合蛋白后免疫家兔制备多克隆抗体,并检测不同的抗血清对噬菌体增殖的影响。结果显示,经SDS-PAGE及Western blot鉴定证实得到含有GST标签的67000、53000的gp35、gp38重组融合蛋白;抗噬菌体Bp7尾丝蛋白gp38与gp35的抗血清均能明显抑制噬菌体Bp7的增殖,而其中gp38的抗血清更是能完全抑制Bp7增殖,证明gp35、gp38参与噬菌体Bp7的宿主识别过程。
The aim was to study the function of gp35 and gp38 in the host recognition of phage Bp7. The genes of tail fiber protein gp35 and gp38 were amplified and cloned into recombinant plasmid,which was transformed into BL21 and then induced by IPTG. The polyclonal antibodies against GST · gp35 and GST · gp38 were prepared to study the effection on the phage reproduction. The results of SDS-PAGE and Western-blot showed that 67 000 and 53 000 GST-tagged fusion protein were obtained successfully. The polyclonal antibodies to gp35 and gp38 could inhibit the reproduction of Bp7 obviously,while the polyclonal antibody to gp38 could inhibit the reproduction of Bp7 completely. The results showed that gp35 and gp38 participated in the process of host recognition of Bp7.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第5期722-726,共5页
Chinese Journal of Veterinary Science
基金
山东省自然科学基金资助项目(ZR2009DM009)
关键词
多价噬菌体
尾丝蛋白
原核表达
宿主识别
polyvalent phage
tail fiber protein
prokaryotic expression
host recognition
作者简介
颜晨（1987-），女，硕士。
与第一作者同等贡献
通讯作者，Email：renren0228@sina．com；
