摘要
目的对市场上销售的5种HIV抗体诊断试剂的质量进行评估。方法使用5种酶联免疫(ELISA)试剂检测200份血清标本中HIV抗体,其中包括100份已知结果样本和100份高危人群血清标本,并对可疑样本进行WB确证。结果 5种试剂的敏感性、特异性、假阳性率、假阴性率、功效率、阳性预示值(PPV)、阴性预示值(NPV)分别在97.33%～98.67%、97.60%～99.20%、0.8%～2.4%、1.33%～2.67%、97.50%～99.00%、96.05%～98.67%、98.39%～99.20%范围内。结论艾滋病毒抗体检测试剂质量参差不齐,试剂质量需进一步提高。
OBJECTIVE To evaluate five screening reagents which were used in Zhangjiakou for detecting antibodies of HIV. METHODS Five elisa screening reagents were used to detect the samples, including 100 known samples and 100 unknown samples. And suspicious samples were conformed by WB test. RESULTS The sensitivities of all the screening reagents were ranged from 97.33%-98.67%, the specificities 97.60%-99.20%, the total consistence rates 97.50%-99.00%, the false positive rates 0.8%-2.4%, the false negative rates 1.33%-2.67%, the PPV 96.05%-98.67% and the NPV 98.39%-99.20%. CON- CLUSION There is wide gap among all of reagents. In a word, the quality of reagents made in China should be further im- proved.
出处
《现代预防医学》
CAS
北大核心
2013年第10期1947-1948,共2页
Modern Preventive Medicine
关键词
艾滋病病毒
抗体
试剂
质量评估
HIV
Antibody
Screening reagent
Evaluation of quality
作者简介
刘琪琛（1974-），男，本科，主治医师，研究方向：艾滋病防治
