摘要
从蚂蚁中分离纯化出抗菌肽并对其部分生物活性进行研究。以长白山野生红蚂蚁为原料,采用乙醇浸提法进行粗提,再利用加热-层析法分离纯化,得到具有抑菌活性的小分子多肽,进行相对分子质量和抑菌活性的测定。结果表明:经乙醇浸提和加热层析得到的蚂蚁抗菌肽纯度较高,SDS-PAGE电泳表现为单一条带,其分子质量为3.746kD;蚂蚁抗菌肽对大肠埃希氏菌、金黄色葡萄球菌、肠炎沙门氏菌、枯草芽孢杆菌均有抑菌作用,最小抑菌质量浓度分别为0.325、0.65、0.1625、0.325mg/mL;蚂蚁抗菌肽表现出较高的热稳定性。
Objective： To isolate antimicrobial peptide from ants and explore its biological activity.Methods： Wild red ants from Changbai Mountain were used as the raw material,ethanol-leaching method was used to extract the crude peptide and heating-column chromatography was used for purification.The relative molecular mass and the bacteriostatic activity of small molecule polypeptide with antibacterial activity were determined.Results： The antimicrobial peptide obtained from this method revealed high purity.Relative molecular mass of the antimicrobial peptide was determined to be 3.746 kD.The antimicrobial peptide from ants had bacteriostatic capacity to Escherichia coli,Staphylococcus aureus,Salmonella enteritidis and Bacillus subtilis with minimum antibacterial concentrations of 0.325,0.65,0.1625 mg/mL and 0.325 mg/mL.Moreover,the antimicrobial peptide from ants exhibited high thermal stability.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第9期70-73,共4页
Food Science
基金
吉林省重大科技资助项目(20060208)
关键词
蚂蚁
抗菌肽
分离纯化
抑菌活性
ant
antimicrobial peptide
isolation
antimicrobial activity
作者简介
苏翔（1987-），男，硕士研究生，研究方向为食品生物化学与功能食品。E-mail：425261105@qq．com
通信作者：陈晓平（1963-），男，教授，博士，研究方向为食品生物化学与功能食品。E—mail：13578883136@163．com
