摘要
以市售盆栽菊花为试材,研究了离体条件下不同外植体来源、外植体大小和不同激素浓度培养基对菊花愈伤组织诱导的影响。结果表明:2mm长的茎段作为外植体诱导愈伤组织分化最佳;愈伤组织诱导的最佳培养基为MS+6-BA 2.0mg/L+KT 0.5mg/L,出愈率可以达到100%,且愈伤组织生长良好,适于进行根苗分化。
Taking market Chrysanthemum rnorifolium L. as material,the effect of different explants,size of explants and culture media with different kinds and concentrations of hormones on callus induction of Chrysanthemum morifolium L. were studied in vitro. The results showed that 2 mm stem sections as explants was suitable for callus induction. The best callus induction medium was MSA-IBA 2. 0 mg/L＋KT 0. 5 mg/L,with induction rate 100%. Callus developed well,and suitable for seeding differentiation.
出处
《北方园艺》
CAS
北大核心
2013年第9期132-133,共2页
Northern Horticulture
关键词
菊花
影响因素
愈伤组织
Chrysanthemum morifolium L.
influence factors
callus
作者简介
李翠香（1973-），女，硕士，实验师，研究方向为植物生物技术。E-mail：licuixiang2005@126．com.
