摘要
通过对溶杆菌属细菌Lysobacteryanansis sp.nov.胞外蛋白上样量和聚焦程序两个方面进行双向凝胶电泳条件的优化,分析了其对溶杆菌Lysobacteryanansis sp.nov.胞外分泌蛋白的双向凝胶电泳图谱的影响。结果表明,150μg的上样量和聚焦程序Ⅱ获得的双向电泳图谱蛋白点数较多且清晰。同时,建立了溶杆菌属细菌Lysobacteryanansis sp.nov.胞外和胞内蛋白双向电泳图谱,经过硝酸银染色和PDQuest图像分析检测到了100种胞外蛋白和204种胞内蛋白。
In this study, we separated the extracellular protein excreted by Lysobacter yanansis sp. nov. through the two-dimensional （ 2-D ） electrophoresis, and analyzed the effect of isoelectric focusing programs and loading volumes on 2-D proteomic maps. The results showed that a clearly 2-DE map was obtained when the 150 μg loading volumes and isoelectric focusing programsⅡ were used. Furthermore, 2-D proteomic maps of extract intracellular and extracellular protein from Lysobacter yanansis sp. nov. were constructed. The separated protein spots in gels were analyzed by PDQuest. 100 spots of extracellular proteins and about 204 spots of intracellular proteins had been separated by 2-DE.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第4期140-146,共7页
Biotechnology Bulletin
基金
国家自然科学基金项目(31101481)
中央高校基本科研业务费专项资金(GK200902032)
2012年度陕西师范大学实验技术研究立项项目(SYJS201213)
陕西师范大学青年项目(201001005)
陕西师范大学2011年校级大学生创新实验计划项目(cx11072)
关键词
溶杆菌属
双向电泳
生物防治
Lysobacter
Two-dimensional electrophoresis （ 2-DE ）
Biodefence
作者简介
王斐斐，女，硕士，研究方向：微生物蛋白质组学；E—mail：wangfeifei488@163．com.
通讯作者：崔浪军，男，副教授，硕士生导师，研究方向：药用植物内生菌的开发与利用；E-mail：ljcui@snnu．edu．cn
