摘要
目的采用沙土鼠全脑缺血再灌注模型动态观察丁基苯酞(NBP)对脑缺血再灌注损伤后p-ERK表达的影响。方法健康雄性沙土鼠分为3组:假手术组、再灌注组、NBP治疗组,采用Nissl染色和Western blot法分别于1、3、7d测沙土鼠脑缺血再灌注后海马CA1区神经锥体细胞的变化和p-ERK的表达。结果与假手术组对比,再灌注组神经元数量大量减少,NBP治疗组神经元数量相对较多。NBP治疗组较再灌注组p-ERK升高明显,差异有统计学意义(P<0.01)。结论 NBP能通过调节p-ERK的表达起到改善沙土鼠脑缺血再灌注损伤的作用。
Objective To observe the effect of NBP on the impact of p-ERK in gerbil after cerebral ischemia reperfusion（I/R） injury. Methods Healthy male gerbils were randomly divided into 3 groups （CON, I/R, I/R＋NBP）. Measurement of the gerbil global cerebral ischemia reperfusion in hippocampus CA1 pyramidal cells and the changes of p-ERK expression were conducted by Nissl staining and Western blot method after 1,3,7 days. Results A large number of neurons reduced in the reperfusion group. The number of neurons in the NBP treatment group was more than that of reperfusion group. P-ERK rose in reperfusion group, which showed significant difference（P〈0.01）. Conclusion NBP may protect the brain tissue in gerbil after cerebral ischemia reperfusion injury by regulating p-ERK expression.
出处
《重庆医学》
CAS
CSCD
北大核心
2013年第12期1377-1379,共3页
Chongqing medicine
作者简介
赵丽薇（1980-），主治医师，硕士，主要从事缺血性脑血管病研究。
