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SIR方法研究真菌、细菌对森林土壤N_2O贡献量的进展

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摘要 土壤硝化、反硝化是产生有害温室气体N2O主要的生物化学过程。除了原核细菌外还发现真核生物真菌(包括菌根真菌)也能参与土壤的硝化和反硝化过程并排放N2O。基质诱导抑制呼吸法(SIR)是目前研究真菌与细菌对N2O排放通量贡献的主要方法。该方法应注意以下几点:(1)诱导剂的选择应该根据不同土壤的理化性质选择,一般选择的诱导剂为葡萄糖,也有选择蛋白胨作为诱导剂。(2)预实验确定饱和诱导剂的量。(3)抑制剂应根据土壤理化性质进行选择,一般选择的抑制剂为放线菌酮和链霉素。(4)确定IAR,最大抑制率的时间。(5)真菌、细菌对N2O排放贡献量时间段的确定。
作者 章伟
机构地区 福建师范大学
出处 《安徽农学通报》 2013年第5期19-20,共2页 Anhui Agricultural Science Bulletin
作者简介 章伟(1988-),男,在读研究生,研究方向:森林碳氮循环。
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参考文献11

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