摘要
目的:探讨电针"风府""太冲"穴治疗帕金森病的的作用机制。方法:将32只健康雄性SD大鼠随机分为4组。模型组:采用颈背部皮下注射鱼藤酮(2 mg/kg,溶解于葵花油,浓度2 mg/mL)法制备PD模型,1次/d,连续28 d。假手术组:与模型组相同部位注射等量的不含鱼藤酮的葵花油。电针组:造模成功后大鼠,取"风府"、"太冲"两穴,采用连续波,频率2 Hz,强度1 mA,通电20 min,1次/d,连续14 d。正常组:不做任何处理,正常喂养。对各组进行行为学观察,采用免疫组化法检测大鼠中脑黑质区COX-2及PGE2的表达。结果模型组大鼠出现典型的PD行为学改变,中脑黑质区COX-2、PGE2的阳性细胞数较正常组、假手术组明显升高(P<0.01),电针组较模型组明显降低,差异具有统计学意义(P<0.01)。结论:电针可通过降低中脑黑质区炎性介质COX-2、PGE2的表达,减少炎性反应,对PD模型大鼠多巴胺能神经元起到一定的保护作用。
Objective ： To explore the mechanism of electroacupuncture （EA） at Fengfu （ DU16 ） and Taichong （ LR3 ） in Parkinson disease （PD） rats. Methods ：32 rats were randomly divided into 4 groups. Model group ： the rats were established by subcutaneous injection of rotenone （2 mg/kg, dissolved in sunflower oil, concentration 2 mg/mL）to induce the rats model of PD, once a day, continuous for 28 days. Sham operation group：the rats were injected as the model group, the same parts of the same amount of excluding rotenone sunflower oil. EA group：the rats with PD were treated with electroacupuncture at Fengfu（DU16） and Taichong （LR3） with continuous -wave ,2 Hz and 1 mA for 20 minutes, once a day for 14 days. Normal group：the rats received no treat- ment, normal feeding. The expressions of COX -2 and PGE2 in the substantia nigra were detected by immunohistochemistry. Results ： Compared with normal group and sham operation group, the positive neurons counts of the COX - 2 and PGE2 were significantly increased in model group（ P 〈 0. 01 ）. After the EA treatment, the expressions of COX -2 and PGE2 in EA group were significantly decreased in comparison with model group（P 〈 0.01 ）. Conclusion：EA therapy can play a protective role to PD rats of dopaminergic neurons by reducing the expression of inflammatory mediaors COX -2 and PGE2 in substantia nigra.
出处
《辽宁中医杂志》
CAS
2013年第4期800-802,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(30973787
30973809)
浙江省"重中之重"学科(针灸推拿学)开放基金项目(ZTK2010A10)
湖北省卫生厅中医药中西医结合科研项目(2010Z-Z01)
湖北省教育厅科研项目(D20112002)
作者简介
马骏（1964-），女，湖北武汉人，教授，博士，研究方向：针灸防治脑病及神经系统疾病的研究。
通讯作者：王述菊（1977-），女，湖北南漳人，副教授，博士，研究方向：针灸防治脑病及神经系统疾病的研究。E-mail：gradfu@sina.com
