摘要
收集不同辣椒品种,采用对硝基苯-α-D-吡喃葡萄糖苷(p-nitrophenyl-α-D-glucopyranoside,pNPG)对辣椒叶70%乙醇提取物进行α-葡萄糖苷酶抑制活性测定,并通过动物糖负荷试验研究辣椒叶提取物的降糖效果.结果表明:辣椒叶品系间对α-葡萄糖苷酶抑制活性差异显著;同辣椒果实提取物相比,辣椒叶提取物对α-葡萄糖苷酶抑制活性高达60%,是辣椒果实提取物的10倍左右;辣椒叶提取物对不同来源α-葡萄糖苷酶表达不同,对于动物来源的蔗糖酶和麦芽糖酶的抑制具有不同程度的表达,而对于微生物来源的酵母(Saccharomycescerevisiae)和嗜热芽孢杆菌(Bacillus stearothermophillus)的α-葡萄糖苷酶抑制均没有表达;动物糖负荷试验表明,辣椒叶提取物具有较强的降血糖活性.
Capsicum annuum is one of the world largest consumptive vegetables. As the biggest producer, consumer and exporter of C. annuum in the world, the development of China capsicum industry has an important influence on the development of global C. annuum industry. The C. annuum industry mostly focuses on its fruit, while C. annuum leaves, as a kind of easily wasted vegetables, which are rich in resources and nutrients, have not yet been widely developed. In order to better develop C. annuum leaves, we explore the α-glucosidase inhibitory activity using 70% ethanol extracts of C. annuum leaves. The p-nitrophenyl-α-D-glucopyranoside （pNPG） method was adopted to determine the inhibitory activity of C. annuum leaf extracts against α-glucosidase. The results showed that the α-glucosidase inhibitory activity of C. annuum leaf extracts was up to 60%,which was 10 times more than its fruit, and there were significant differences in the α-glucosidase inhibitory activity among different cuhivars. As to different target enzymes, C. annuum leaf extract showed different activity, for example, it could inhibit the activity of sucrase and mahase from animal, but it had no effect for microbial enzyme. The C. annuum leaf extract were validated with a strong hypoglycemic activity by animal glucose load test. In conclusion, The C. annuum leaf extract has high inhibition against α-glucosidase and can be used for the development of a new type of hypoglycemic food.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2013年第2期173-177,共5页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
"食品贮藏加工及质量安全控制工程技术研究中心"与"辽宁省食品安全重点实验室"开放课题资助项目(LNSAKF2011001)
浙江省教育厅科研资助项目(N20100114)
作者简介
通信作者（Corresponding author）：李春英，E—mail：cyli@zju．edu．cn；
通信作者（Corresponding author）：励建荣，E-mail：lijianrong@zjgsu．edu．cn
