摘要
用三氧化硫吡啶复合物将β-环糊精与环氧氯丙烷反应得到的β-环糊精聚合物进行磺酸化,得到磺酸化的β-环糊精聚合物,并对其进行了红外光谱和1H NMR表征。将磺酸化β-环糊精聚合物作为手性添加剂,用于毛细管电泳手性药物拆分中,在区带电泳模式下,对5种药物特布他林、山莨菪碱、奥美拉唑、扑尔敏、氧氟沙星进行拆分,并考察了缓冲溶液pH值、运行电压、手性添加剂浓度、有机溶剂对手性拆分的影响。1H NMR分析结果表明:此聚合物为不均一取代的磺酸化β-环糊精;毛细管电泳结果表明,此聚合物具有良好的拆分效果,在磺酸化β-环糊精聚合物浓度为5 g/L,运行电压15 kV条件下,对特布他林、扑尔敏的分离度达到3.0以上;对于山莨菪碱的两对对映体均可实现良好拆分,分离度达到2.0以上。
The β-cyclodextrin polymer obtained by the reaction of β-cyclodextrin with epicholrohydrin was sulfated with sulfur trioxide-pyridine complex to obtain a sulfated β-cyclodextrin polymer.The sulfated β-cyclodextrin polymer was characterized with IR and 1H NMR.As chiral additive,the sulfated β-cyclodextrin polymer was used in capillary zone electrophoresis for enantioseparation.The effects of pH value,concentration of additive,running voltage and organic solvent on the enantioseparation were investigated.The results of 1H NMR indicated that the substitution degree of the polymer was not uniform.The results of capillary electrophoresis showed that the sulfated β-cyclodextrin polymer had good chiral separation ability although it was not uniform substituted.Five racemic chiral drugs,terbutaline,anisodamine,chlorphenamine,omeprazole,and ofloxacin,were successfully separated into their isomers.Under the conditions of 5 g/L sulfated β-cyclodextrin polymer and 15 kV running voltage,the resolutions were more than 3.0 for terbutaline and chlorphenamine,and the resolutions for anisodamine enantiomers were both above 2.0.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2013年第4期559-564,共6页
Chinese Journal of Analytical Chemistry
基金
北京中医药大学自主选题项目(No.2011-JSBZZ-JS047)资助
关键词
磺酸化β-环糊精聚合物
手性分离
毛细管电泳
Sulfated β-cyclodextrin polymer
Chiral separation
Capillary electrophoresis
作者简介
E—mail：dyuance@yahoo．com．cn
