摘要
提出了液相色谱-串联质谱法同时测定浓缩胡萝卜汁中苏丹橙G、苏丹红G、苏丹红7B、苏丹黄、苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ等8种苏丹类染料含量的方法。样品经正己烷提取,于39℃旋转蒸发至近干,氮气吹干后用1mL乙腈和0.1%(体积分数,下同)甲酸(1+1)混合液溶解。采用Hypersil GOLD色谱柱(50mm×2.1mm,1.9μm)为分离柱和以不同比例的0.1%甲酸和甲醇混合液为流动相作梯度淋洗,采用多反应监测正离子模式监测。8种苏丹类染料的质量浓度均在30～100μg.L-1范围内呈线性,测定下限(10S/N)在5～30μg.kg-1之间。在3个浓度水平下做加标回收试验,回收率在72.0%～96.0%之间,测定值的相对标准偏差(n=6)在6.2%～9.6%之间。
LC-MS/MS was applied to the determination of 8 Sudan dyes,i.e.,Sudan orange G,Sudan red G,Sudan red 7B,Sudan yellow,Sudan red Ⅰ,Sudan red Ⅱ,Sudan red Ⅲ and Sudan red Ⅳ,in concentrated carrot juice.The sample was extracted by n-hexane,concentrated to near dryness in a rotary evaporator at 39 ℃,and evaporated to dryness by N2-blowing.The residue was dissolved with 1 mL of a mixed solvent of methanol and 0.1%（φ） formic acid（1＋1）.Hypersil GOLD（50 mm×2.1 mm,1.9 μm） column was used as chromatographic column,and mixtures of methanol and 0.1%（φ） formic acid in various ratios were used as mobile phase in the gradient elution;positive electrospray ionization as well as multiple reaction monitoring mode was used in the detection.Linear relationships between values of peak area and mass concentration of 8 Sudan dyes were kept in the same range of 30-100 μg·L-1,with lower limits of determination（10S/N） in the range of 5-30 μg·kg-1.Tests for recovery were made by addition of standard solutions at 3 different concentration levels,values of recovery found were in the range of 72.0%-96.0%,with values of RSD′s（n=6） in the range of 6.2%-9.6%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2013年第3期306-309,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
作者简介
倪永付（1983-），男，山东微山人，工程师，研究方向为食品检验。E-mail：niyongfu@126．com
