摘要
目的 :进一步研究脑血管痉挛的发生机理 ,为临床治疗服务。方法 :采用 1 4只成年家犬 ,实验组 9只 ,对照组 5只 ,通过 2次枕大池注血法建立蛛网膜下腔出血 (SAH)模型。观察痉挛血管的自由基变化、血管活性、血管造影像、超微结构变化。另外 2 0只犬用来观察血管扩张剂的作用。结果 :实验组较对照组自由基含量高 ,血管活性降低 ,血管狭窄 ,管壁损害重。结论 :尼莫地平对急性痉挛有效 ,对慢性痉挛无效 ;慢性血管痉挛是以管壁结构性狭窄为特点。
? Objective:To further study the pathogenesis of cerebral vasospasm following subarachnoid hemorrhage to serve the clinical treatment.Methods:In this research,canine double SAH model was established by two successive injections of cisternal blood administered 72 hours apart.Angiogram,vessel function,free radical activity and pathological changes in vessel wall survived until sacrifice on the 7th day after first injection were studied in all animals ( n =9 for hemorrhage,and n =5 for control group).Results:Angiograms of basilar arteries (BA) showed:At 30th minute,1st hour and 7th day after first injection,an average arterial diameter was 68.3%±15.6% ( P <0 01),66.8%±13.8% ( P <0 01) and 52.7%±19% ( P <0 001) respectively.At 30th minute after nimodipine (0.1 mg/kg,intravenously, n =13) was given at 30th minute and on 7th day after first injection of blood,the BA′s diameter changed from 59%±13.6% to 96.2%±12.1% ( P <0 01) and 60.6%±13.6% to 72.4%±19.6% ( P >0 05) of the basiline respectively.The preventive consecutive application of nimodipine (40mg by oral administration,every 6 hours untill the last day of study, n =7) had no effects on chronic CVS.Rings of basilar arteries obtained from SAH group contracted much more weakly than those from control group in response to vasoconstrictors ( P <0 01).MDA of BA′s wall was much more in SAH group than in control group ( P <0 01),and MDA content was consistent with the vessel wall injuries.The pathological study of BA′s wall confirmed degenerative changes in the tunica intima and media,subendothelial and medial thickening,partial myonecrotic changes,and the leukocytic infiltration in the tunica adventitia.Conclusions:①Nimodipine can reverse acute vasospasm,but it shows no effects on chronic vasospasm.②High concentration of lipid peroxides in chronic constricted vessel wall relates to vessel wall′s damage.③Chronic CVS is demonstrated in structural constriction involved in vessel wall′s damage following SAH. 〔
出处
《白求恩医科大学学报》
CSCD
2000年第5期519-522,共4页
Journal of Norman Bethune University of Medical Science
作者简介
Liu Baiyun(1963-),male,Changchun Jilin,Assistant professor,MD.Ph.D,Majors in experimental and clinica1 study of cerebral vasospastn.
