摘要
目的:研究六亚甲基二乙酰胺(hexamethylene bisacetamide,HMBA)对人卵巢癌HO8910细胞增殖和细胞周期的影响。方法:采用MTT法测量不同浓度的HMBA(1.0、3.0、5.0、7.0、9.0mmol/L)对人卵巢癌细胞HO8910增殖的影响,流式细胞术观察HMBA作用后细胞周期的变化。结果:MTT显示HMBA处理后的HO8910细胞生长抑制作用随着浓度的增加逐渐增强(r=0.959 8,P<0.05);流式细胞仪检测结果显示,空白对照组HO8910细胞G_1期比例为(67.15±0.57)%,S期为(23.55±0.11)%,G_2期为(9.31±0.48)%;5.0 mmol/L HMBA作用后的HO8910细胞,G_1期比例为(73.64±1.83)%,S期为(17.70±0.74)%,G_2期为(8.66±1.10)%,细胞明显被阻滞于G_1期(P<0.05)。结论:HMBA对人卵巢癌HO8910细胞增殖具有抑制作用。HMBA能将HO8910细胞周期阻滞于G_0/G_1期,诱导HO8910细胞向正常细胞分化,促进细胞凋亡。
Objective：To study the effects of hexamethylene bisacetamide （HMBA） on cell proliferation and cycle in human ovarian cancer HO8910 cells. Methods： The MTT technique was employed to assess the effects of different concentrations of HMBA （ 1.0, 3.0, 5.0, 7.0 and 9.0 mmol/L） on ovarian cancer HO8910 cell proliferation. Changes in cell cycle were determined by flow cytometry. Results： The growth of HO8910 cells was increasingly suppressed as the concentration of HMBA increased （ r = 0. 959 8, P 〈 0.05 ）. Flow eytometry showed significant changes in the proportion of cell cycles following administration of 5.0 mmol/L HMBA（[67.15±0.57]% vs. [73.64±1.83]% for phaseG1; [23.55±0.11]% vs. [17.70±0.741% for phase S and [9.31± 0.481% vs. [ 8.66 ± 1. 10] for phase G2 ）, suggesting that the cell proliferation was inhibited at phase G1. Conclusion： HMBA inhibits proliferation of H08910 cells as a consequence of stagnation at stages G0/G1 and induction of differentiation to normal cells leading to cell apoptosis.
出处
《广州医学院学报》
2012年第6期26-28,共3页
Academic Journal of Guangzhou Medical College
关键词
HMBA
HO8910人卵巢癌细胞
细胞增殖
细胞周期
hexamethylene bisacetamide
human ovarian cancer HO8910 cells
cell proliferation
cellcycle
作者简介
叶璐（1976-），女，副主任医师，本科。研究方向：妇科肿瘤的临床与基础研究。通讯作者：E-mail：aabb97@163．com
