摘要
目的:研究云南鼠尾草茎叶的化学成分,寻找抗血管生成活性成分,综合开发药用植物资源。方法:利用溶剂超声提取,硅胶柱色谱、凝胶柱色谱、MCI柱色谱等分离手段进行分离纯化,根据化合物理化性质和波谱数据对其进行结构鉴定,通过鸡胚尿囊膜模型(CAM)进行活性筛选。结果:从提取物中共分离得到7个化合物,分别被鉴定为(+)桉油烯醇(1)、5,7,4'-三羟基二氢黄酮(2)、β-香树素(3)、3β-羟-12-乌苏烯(4)、马斯里酸(5)、熊果酸(6)、3-氧-12-烯-28-乌苏酸(7)。抗血管生成实验表明化合物5,6在鸡胚尿囊膜模型中表现出较好抗血管生成作用。结论:化合物1,2,5,6首次从该植物中分离得到,并首次发现化合物5(齐墩果烷型),6(乌苏烷型)在0.8 nmol/egg的剂量下就具有抑制血管生成的活性,且呈剂量依赖性。
Present study was focused on the chemical constituents of the stems and leaves of Salvia yunnanensis C . H. Wright and their anti-angiogeneic activities. The compounds were isolated by column chromatography over silica gel and Sephadex LH-20, and other isolation techniques. Their structures were elucidated on the basis of spectral analysis and chemical evidences. Their anti-angiogeneic activities were evaluated by the chicken chorioallantoic membrane （CAM） neovascularisation model. Seven compounds were separated and identified as （ ＋ ）-spathulenol（1）, 5,7,4＇-trihydroxyflavanone（2）, β-amyrin（3）, 3β-hydroxy-12-ursene（4）, 2a,3β- dihydroxyursa-12-en-28-oic acid （5）, ursolic acid （6） and 3-oxo-12-ursen-28-oic acid （7）. Compounds 1, 2, 5 and 6 were obtained from this plant for the first time. Compounds 5 （an oleanane compound） and 6 （an ursane compound） could inhibit angiogenesis sig- nificantly in a dose-dependent manner.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第6期835-838,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30960042)
云南省应用基础研究计划项目(2009ZC047M)
作者简介
向诚，讲师，主要从事中药化学成分活性研究工作，Tel：（0871）65920738，E—mail：xeheng0871@163．com
[通信作者]李宝才，教授，主要从事天然产物医药开发研究工作，Tel：15912531421，E．mail：baocaili@hotmail．com
