摘要
利用人工智能模拟霜箱,设8个温度(18、–1、–2、–3、–4、–5、–6、–7℃),对抗寒性不同的两个仁用杏(Prunus armeniaca Linn.)品种雌蕊的过氧化氢(H2O2)含量和抗坏血酸—谷胱甘肽(AsA-GSH)循环进行了研究。结果表明,低温胁迫增加了仁用杏雌蕊H2O2含量,–7℃时抗晚霜品种‘围选1号’和晚霜敏感品种‘龙王帽’分别为对照(18℃)的5.49倍和7.22倍。低温期间,‘围选1号’雌蕊AsA等酶含量及APX等酶活性均高于‘龙王帽’,–7℃时,‘围选1号’雌蕊AsA、MDHA、DHA、GSH和GSSG含量比对照分别下降了66%、69%、48%、52%、53%,‘龙王帽’下降了78%、79%、59%、66%、65%,–7℃时,‘围选1号’雌蕊APX、MDHAR、DHAR、GPX、GR活性、AsA/DHA、GSH/GSSG分别是对照的2.87倍、1.07倍、1.42倍、1.60倍、1.20倍、65%和1.03倍,‘龙王帽’是对照2.41倍、96%、1.13倍、1.08倍、98%、54%、97%。结果表明‘围选1号’雌蕊在低温期间有较高的AsA-GSH循环效率,可有效抑制H2O2的积累,这可能是其抗晚霜能力较强的重要原因之一。
The peroxide （H202） content and ascorbate （AsA） -glutathione （GSH） cycle in pistil of two kernel apricot （Prunus armeniaca Linn.） cultivars under different low temperature （ 18, - 1, - 2,- 3, - 4, - 5, - 6, - 7 ℃ ） were studied using the artificial climate chamber. The results show that H202 contents in kernel apricot pistilincreased under the low temperature stress, at- 7 ℃, the amount of＇Weixuan 1＇ resistant to cold stress was 5.49 times more than control （18 ℃ ）, the amount of ‘ Longwangrnao＇ susceptible to cold stress was 7.22 times more than the control. The content of enzymelike AsA and so on and activity of enzyme like APX and so on in ‘Weixuan 1＇ pistil were higher than those in ‘Longwangmao＇ under low temperature, at- 7 ℃, the contents ofAsA, MDHA, DHA, GSH andGSSG were decreased by 66%, 69%, 48%, 52%, 53% compared to the control （ 18 ℃ ） in ＇Weixuan 1＇ pistil, were decreased by 78%, 79%, 59%, 66%, 65% compared to control （ 18 ℃ ） in ‘Longwangmao＇pistil. At - 7 ℃, the activity ofAPX, MDHAR, DHAR, GPX, GR, GSH/GSSG were 2.87, 1.07, 1.42, 1.60, 1.20, 1.03 times more than control （ 18 ℃ ） respectively. AsA/DHAwas 65% of the controlin ‘Weixuan 1＇ pistil. The activity ofAPX, DHAR, GPX were 2.41, 1.13, 1.08 times more than control （ 18 ℃ ）. MDHAR, GR, AsA/DHA, GSt-I/GSSG were 96%, 98%, 54%, 97% of the control respectivelyin ‘Longwangmao＇ pistil. The results indicate that ‘Weixuan 1＇ pistil could maintain efficient metabolism of AsA-GSH cycle, inhibit the accumulation of H202 effectivity, this might be one of important reasons forhigher cold tolerance of ‘Weixuan 1＇
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第3期417-425,共9页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(C041102)
河北省自然科学基金项目(C2009000552)
河北省科技支撑计划项目(09230603D
06220117D216)
作者简介
通信作者Author for correspondence（E-mail：lbg888@163．com）
