摘要
目的探讨新诊断2型糖尿病(T2DM)患者血清nesfatin-1、apelin与胰岛素抵抗(IR)的关系。方法选取2011年6月—2012年2月在我院就诊的新诊断2型糖尿病患者60例(T2DM组)及同期体检健康者30例(NC组)。采用酶联免疫吸附法(ELISA法)测定两组受检者空腹血清nesfatin-1、apelin水平,同时测定空腹血糖(FPG)、空腹胰岛素(FINS)、血脂水平。应用稳态模型评估的胰岛素抵抗指数(HOMA-IR)、胰岛素敏感性指数(ISI)评价胰岛素敏感性,用稳态模型评估的胰岛β细胞功能指数(HOMA-β)评价胰岛β细胞功能。采用Spearman秩相关及多元线性逐步回归分析观察nesfatin-1、apelin与糖代谢、IR的关系。结果 T2DM组患者血清nesfatin-1、apelin水平均高于NC组,差异有统计学意义(P<0.01);且二者均与体质指数(BMI)、空腹血糖(FPG)、三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、空腹胰岛素(FINS)、HOMA-IR呈正相关(P<0.05),与高密度脂蛋白胆固醇(HDL-C)、HOMA-β、ISI呈负相关(P<0.01),且nesfatin-1与apelin呈正相关(P<0.01)。多元线性逐步回归分析发现,apelin是nesfatin-1的独立影响因素,常数项为0.018,β=0.574,P<0.01。结论新诊断2型糖尿病患者血清nesfatin-1、apelin水平较健康者显著升高,并与IR存在一定的相关性。提示nesfatin-1、apelin可能参与了T2DM及IR的病理生理过程。
Objective To investigate the relationship between nesfatin - 1, apelin and insulin resistance in newly di- agnosed type 2 diabetes mellitus （T2DM） patients. Methods A total of 60 T2DM patients （ T2DM group） and 30 non - diabet- ic controls （ NC group） admitted to our hospital from June 2011 to February 2012 were recruited. Fasting plasma nesfatin - 1 and apelin levels were assayed by enzyme - linked immunosorbent assay （ELISA） , At the same time, the fasting plasma glucose （ FPG）, fasting insulin （FINS） and blood lipid indexes were detected. Homeostasis model assessment insulin resistance index （HOMA -IR） and insulin sensitivity index （ISI） were used to evaluate insulin sensitivity, while homeostasis model assessment of pancreatic islet β cell function index （ HOMA - β） were used to evaluate islet β cell function. The Spearman rank correlation and multiple linear stepwise regression analysis were used to test the correlation between nesfatin - 1, apelin and glucose metabo- lism and insulin resistance. Results The levels of serum nesfatin - 1 and apelin in T2DM group were significantly higher than the NC group （P 〈0. 01 ）, and the levels of both nesfatin -1 and apelin were positively correlated with body mass index （BMI）, FPG, triglyceride （TG）, cholesterol （ TC）, low - density liporotein cholesterol （ LDL - C）, FINS and HOMA - IR （ P 〈 0.05 ）, but were negatively correlated with high- density liporotein cholesterol （HDL- C）, ISI and HOMA -β （P 〈 0.01 ）. The nesfatin - 1 and apelin were positively correlated with each other （ P 〈 0. 01 ） . Multiple linear stepwise regression analysis showed that apelin was the independent correlative factor for nesfatin - 1 （ constant term = 0. 018, β= 0. 574, P 〈 0. 01）. Con- clusion Significant increases of serum nesfatin - 1 and apelin are found in patients with T2DM patients compared with the normal controls. The increase is to some extent associated with insulin resistance, indicating that nesfatin - 1 and apelin may be involved in the occurrence and development of T2DM and IR.
出处
《中国全科医学》
CAS
CSCD
北大核心
2013年第5期493-495,共3页
Chinese General Practice
作者简介
通讯作者：潘佳秋，154002黑龙江省佳木斯市，佳木斯大学附属第一医院内分泌科；E—mail：a119_xu@163．com
