摘要
目的对精浆天门冬氨酸氨基转移酶(AST)及其线粒体同工酶(m-AST)的活性进行检测,并分析AST和m-AST活性与精子膜功能完整性的相关性。方法 122例精液标本,分别检测精浆AST和m-AST活性,用低渗肿胀-伊红Y结合试验(HOS-EY)检测精子头-尾膜完整性,用琥珀酸脱氢酶(SDH)染色法检测精子线粒体SDH,同时分析AST和m-AST活性与精子膜功能完整性的相关性。结果精浆AST与m-AST的活性分别为(238±125)U/L和(147±79)U/L,两者呈明显正相关性(r=0.740,P=0.000)。AST和m-AST活性均与(Ⅰ+Ⅱ+Ⅲ)型精子呈明显正相关性(r=0.443,P=0.013;r=0.691,P=0.000)。AST和m-AST活性均与Ⅳ型精子呈明显负相关性(r=-0.439,P=0.013;r=-0.689,P=0.000)。AST活性与精子SDH阳性率无明显相关性(r=-0.187,P=0.313),而m-AST活性与精子SDH阳性率呈明显负相关性(r=-0.471,P=0.007)。结论精浆AST和m-AST活性与精子膜功能完整性具有相关性,而m-AST活性检测更适合评价精子膜受损程度以及精子线粒体受损状况。
Objective To determine the seminal aspartate aminotransferase（AST） and mitochondrial AST（m- AST） activity,and to analyze their relationship with the functional integrity of human sperm membrane. Methods AST and m-AST activities in 122 samples of seminal plasma were measured. The sperm head membrane and tail membrane integrity were detected by the combined hypoosmotic swelling and eosin Y exclusion test（HOS-EY）. The mitochondrial succinate dehydrogenase（SDH） of sperm was detected by the SDH dyeing method and the correlation between seminal AST or m-AST activity with the functional integrity of human sperm membrane. Results The AST and m- AST activities were （ 238±125 ） U/L and （ 147±79 ） U/L respectively, there was significant positive correlation between them（r= 0. 740,P=0. 000）. Both seminal AST and m-AST activities had obvious positive correlation with type Ⅰ and Ⅱ and Ⅲ sperms（r=0. 443,P=0. 013;r=0. 691,P=0. 000）. Both seminal AST and m-AST activities had obvious negative correlation with type IV sperm（r=- 0. 439, P= 0. 013 ; r= - 0. 689, P= 0. 000）. There was no obvious correlation between AST activity and sperm SDH positive rate（r=-0. 187 ,P=0. 313） ,but seminal m-AST activities had significant negative correlation with sperm SDH positive rate（r =- 0. 471, P = 0. 007）. Conclusion The seminal AST and m-AST activities have significant correlation with the functional integrity of human sperm membrane. The determination of m-AST activity is more suitable for evaluating the damage degree of sperm mem- brane and sperm mitochondria.
出处
《检验医学与临床》
CAS
2013年第5期515-517,共3页
Laboratory Medicine and Clinic
基金
广东省中山市科技计划项目资助课题(NO.20102A061)
关键词
天门冬氨酸氨基转移酶
同工酶
精子膜功能
完整性
精浆
aspartate aminotransferase
isozyme
sperm membrane function
integrity
seminal plas- ma
