摘要
目的:研究NFBD1在鼻咽癌(nasopharyngeal carcinoma,NPC)组织中的表达及其临床意义。方法:采用免疫组化检测40例NPC组织及20例慢性鼻咽炎组织中NFBD1蛋白的表达,实时荧光定量PCR检测34例NPC组织和23例慢性鼻咽炎组织中NFBD1 mRNA的表达并分析其与患者性别、年龄、颈淋巴结转移和临床分期的相关性。结果:NPC组织中NFBD1在蛋白水平(χ2=9.600,P=0.002)和mRNA水平(t=3.206,P=0.002)表达均高于慢性鼻咽炎组织,NFBD1在mRNA水平的表达高低与患者的性别(t=0.937,P=0.356)、年龄(t=0.382,P=0.698)及是否伴有临床颈淋巴结转移(t=1.037,P=0.322)、临床分期(t=0.293,P=0.771)均没有相关性。结论:NFBD1可能参与了NPC的发生,但其与NPC的侵袭转移没有明显联系。
Objective：To investigate the expressions of nuclear factor with BRCT domain 1 （NFBD 1） in nasopharyngeal carcinoma and their clinical significances. Methods：Expressions of NFBD1 protein in 40 cases of nasopharyngeal carcinoma（NPC）tissues and 20 cases of chronic nasopharyngitis tissues were detected by immunohistochemical SP method. Expressions of NFBD1 mRNA in 34 cases of NPC tissues and 23 cases of chronic nasopharyngitis tissues were observed by qRT-PCR and their correlations with patients＇gender,age,cervical lymphnode metastasis and clinic stage were analyzed. Results：Expressions of NFBD1 protein and mRNA were sig- nificantly higher in NPC tissues than in chronic nasopharyngitis tissues（X2=9.6OO,P=O.OO2;t=3.206,P=-O.O02）. Expressions of NFBD1 mRNA in NPC tissues were not significantly correlated with patient＇gender（t=0.937,P=-0.356）, age（t=0.382,P=0.698）, cervical lymphnode metastasis （t= 1.037, P=0.322） and clinic stage （t =0.293, P=0.771）. Conclusions ： Overexpression of NFBD 1 may be involved in the tumorigenesis of NPC, but it is not significantly correlated with nasopharyngeal carcinoma invasion and metastasis.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2013年第2期126-130,共5页
Journal of Chongqing Medical University
基金
重庆市自然科学基金重点资助项目(编号:2012jjB10015)
重庆市卫生局资助项目(编号:2010220)
关键词
NFBD1
鼻咽癌
免疫组织化学
实时荧光定量PCR
nuclear factor with BRCT domain 1
nasopharyngeal carcinoma
immunohistochemistry
real-time fluorescent quantitativePCR
作者简介
邱志利，研究方向：头颈肿瘤的基础与临床。Email：arlyqiu201@sina．com
胡国华，Email：hghcq@sina．com。
