摘要
目的建立较理想的胎鼠神经元细胞体外原代培养方法。方法取13.5 d ICR胎鼠的大脑皮层,经剪碎消化得到单细胞悬液进行培养,观察细胞形态并作PCR及Western blotting等进行神经元相关的基因及蛋白Tuj1和Map2的鉴定。结果细胞生长状态良好,细胞胞体明显,周围有明亮光晕,细胞突触交错形成网络样,通过PCR及Western blotting验证证明所分离培养的细胞是神经元细胞。结论本培养方法简单易行,可获得典型和纯度较高的ICR胎鼠大脑皮层神经元细胞。
Objective To establish a favorable primary culture technique for neurons isolated from embryonic ICR mouse cortical tissues. Methods The cortex of embryonie ICR mice aged 13.5 d was isolated, mechanically dissected and digested, and was proeeeded to culture. The morphology of neurons was observed, and PCR and Western blotting were applied to identify the expression of Tujl and Map2 gene and protein in neurons. Results Cells grew well, with distinct cell body and surrounding bright halation, and there was typical nerve fiber network of synapses. The isolated and cultured ceils were confirmed as neurons by PCR and Western blotting. Conclusion This technique is an easy and practical tool for the primary culture of embryonic ICR mouse cortical neurons with high purity.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2013年第2期249-252,共4页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家自然科学基金(81125003
30900259)
国家高技术研究发展计划项目(2011AA020116)
上海市科委优秀学术带头人项目(12XD1406500)
上海市科委项目(10140900200)
国家重点基础研究发展计划项目(2010CB945200)~~
关键词
神经元
原代培养
ICR胎鼠
疾病模型
neuron
primary culture
embryonic ICR mouse
disease model
作者简介
陈雪松（1983-），女，博士；电子信箱：xsehen1983@gmail．com；
马姬（1973-），女，实验师，学士；电子信箱：maanshan0555@sina．com。
[通信作者]曾凡一，电子信箱：fzeng@sjtu．edu．cn。
