摘要
目的分析HBsAg ELISA法检测阴性献血者的输血HBV感染残余风险,评价其感染状况。方法采用瑞士罗氏诊断公司的Cobas s201核酸检测平台对2011年1月1日至12月31日57 141人份2遍ELISA检测阴性标本进行HBV DNA/HCV RNA/HIV-1,-2RNA三项联合核酸检测(Cobas TaqScreen MPX试剂),检测模式为混样检测+拆分检测,即先进行6标本混样检测,再对检测阳性标本进行拆分检测;对127人份拆分检测阳性标本进行分项鉴别试验及乙型肝炎两对半检测。结果 1)2遍ELISA法共检测标本60 037人份,检出HBsAg阴性标本57 141人份;2)对57 141人份阴性标本进行HBV DNA/HCV RNA/HIV-1,-2RNA三项联检,共检出127人份病毒核酸阳性标本;3)127人份病毒核酸阳性标本进行分项鉴别试验,共检出HBV DNA阳性标本69人份,输血HBV残余风险为0.12%,其定量检测结果以<20U.mL-1为主(51人份,占73.9%);4)69人份HBV DNA阳性标本进行乙型肝炎两对半检测,发现乙型肝炎可疑窗口期感染6人份(占8.7%),隐匿性感染52人份(占75.4%)。结论 HBsAg ELISA法检测阴性献血者的输血HBV感染残余风险依然存在,其感染状况多以隐匿性感染为主,将核酸检测纳入血液筛查常规模式,可降低输血残余风险,提高输血安全。
Objective To analyze the residual risk of transfusion-transmitted HBV infection by ELISA method in HBsAg-negative blood donors,and to assess the infection status.Methods TaqScreen MPX test was performed for the detection of HBV DNA/HCV RNA/HIV-1,-2 RNA on a Cobas s201 system(Swiss Roche Diagnosis Company) in 57 141 HBsAg-negative blood donations.The detection was repeated two times.The detection mode was a combination of 6-sample minipool and individual positive donation test.Then,subentry identification and HBV marker detection were conducted in the 127 positive individual donations.Results A total of 60 037 samples were detected by twice ELISA test and 57 141 of them were negative for HBsAg.Of the 57 141 HBsAg-negative samples,127 were positive for HBV nucleic acid by the detection of HBV DNA/HCV RNA/HIV-1,-2 RNA.Of the 127 nucleic acid-positive samples,69 were positive for HBV DNA by subentry identification.The residual risk of transfusion-transmitted HBV infection was 0.12% with a viral load of <20 U·mL-1(51 samples,73.9%).Of the 69 DNA-positive samples,suspected window period infection was found in 6(8.7%) and occult infection in 52(75.4%) by HBV marker detection.Conclusion HBV remains a risk of transfusion-transmitted infection through ELISA detection in HBsAg-negative blood donors,and occult HBV infection is frequent.Nucleic acid amplification testing of blood donors can reduce the residual risk of transfusion and improve blood transfusion safety.
出处
《南昌大学学报（医学版）》
CAS
2012年第12期35-37,41,共4页
Journal of Nanchang University：Medical Sciences
作者简介
方昌志（1982-），男，学士，中级技师，主要从事抗感染免疫学研究。
通信作者：傅颖嫒，教授，E-mail：hqfyy@126．com。
