摘要
目的监测分析2011年夏季北京地区儿童腹泻主要病原菌,为该地区儿童腹泻病原的快速诊断与防治提供依据。方法收集2011年夏季在该院感染消化科就诊的腹泻患儿粪便标本,提取粪便标本DNA,设计特异性引物建立PCR方法对易导致儿童腹泻的最常见的3种病原菌进行检测,部分阳性结果测序验证,并与以往腹泻病原菌监测结果进行比较分析。结果在259例腹泻患儿中,总病原菌的检出率为69%,其中致腹泻型大肠杆菌属检出率为64%,志贺菌属检出率为15%,沙门菌属检出率为4.2%;男女儿童致病菌检出率、各年龄组间和急慢性腹泻天数比较差异均无统计学意义。本监测结果显示,致腹泻型大肠杆菌属是目前北京市引起儿童夏季腹泻的主要病原菌,其次为志贺菌属。结论 (1)2011年夏季北京地区引起儿童腹泻的主要肠道病原菌种类发生了明显的变化,以致腹泻性大肠杆菌属为主。(2)该研究建立的快速PCR法可检测多种儿童腹泻易感病原菌。
Objective To investigate the main infectious diarrhea pathogens among children in Beijing area in the summer of 2011, and to find out a rapid differential diagnosis method for pathogenic bacteria and provide the basis for prevention and treatment of this disease. Methods Stool samples of children with infectious diarrhea in Department of Gastrointestinal and Infectious in the Affiliated Children＇s Hospital of Capital Institute of Pediatrics were collected from June to August in 2011. Specimens~ DNA was extracted and a specific PCR method was established to detect the most common pathogens which easily to cause the children diar-rhea. The results were sequenced and confirmed,and then compared with the previous studies. Results In the 259 cases with diar rhea,the total detection rate of the pathogen was 69% ,among which the rate of diarrhea cause by enteropathogenic Escherichia coil was 64%,by Shigella bacteria was 15% and by Salmonella bacteria was 4.2%. The rates of pathogenic bacteria detection had non- statistical difference between boys and girls and different age groups, and also no significant difference among the different months in summer. Conclusion Eseherichia coli might be the main pathogen of diarrhea in Beijing this year, followed by Shigella. There could be a significant change about pathogenic bacteria causing children＇s diarrhea in Beijing area in summer. PCR assay, established in this study,could be used for rapid detection of pathogens of children＇s diarrhea.
出处
《国际检验医学杂志》
CAS
2013年第4期432-434,共3页
International Journal of Laboratory Medicine
关键词
微生物学
腹泻
北京
microbiology
diarrhea
Beijing
作者简介
李少丽，女，助理研究员，主要从事医学微生物学研究
通讯作者，E-mail：s.hongmei@263．net
