摘要
目的研究酒精性肝损伤的超微结构变化,以揭示乙醇引起肝脏毒性的发病机制。方法建立酒精性脂肪性肝病大鼠模型,观察肝脏组织病理和超微结构变化,并探讨二者之间的关系。结果电镜下观察模型组可见较多脂滴,线粒体基质密度增高;重者可见线粒体膜断裂,嵴断裂或消失,形态各异;糖原增多,分布不均;细胞核内染色质分布异常。停止乙醇灌胃后病理改变明显减轻。结论随着乙醇给予量的增加和时间的延长,电镜和光镜下的肝脏病理改变呈一致性加重,其中线粒体改变出现的早而且重,戒酒后病变明显减轻。因此戒酒应成为酒精性肝病治疗的首要原则。
Objective To investigate the ultrastructure changes in alcoholic liver injury in order to reveal the pathogenesis of liver toxicity induced by alcohol.Methods By establishing the alcoholic fatty liver rat models,we respectively observed histopathological and ultrastructural changes in liver and explored the relationship between them.Results Under the electron microscope,in the model group,many drops of lipid infiltration were seen and the density of mitochondrial matrix increased.In more severe specimens we even saw the mitochondrial membrane ruptured,the crest broken or disappeared,glucogen increased and was mal-distributed.Meanwhile,the abnormal distribution of chromatin in nucleus could be seen.The pathological changes were obviously relieved after stopping alcoholic gavage.Conclusion As the administered alcohol dose increasing and temporal prolongation,the liver pathological changes under electron and light microscope aggravated uniformly,in which the mitochondria changes occurred early and seriously.Lesions significantly reduced after stop drinking.Above all,alcohol withdrawal should be the most important principle for the alcoholic liver diseases treatment.
出处
《山东大学学报（医学版）》
CAS
北大核心
2012年第8期31-34,45,共5页
Journal of Shandong University:Health Sciences
基金
山东省医药卫生科研重点项目(2007HZ056)
关键词
酒精性肝毒性
动物模型
超微结构
电镜
大鼠
Wistar
Alcoholic liver toxicity
Animal model
Ultrastructural observation
Electron microscope
Rats
Wistar
作者简介
刘莉（1980-），女，主治医师，主要从事酒精性肝病研究。E-mail：doctorliuli@126．com
通讯作者：阎明（1956-），男，博士，主任医师，主要从事酒精性肝病及功能性胃肠病研究。E-mail：ymylh@126．com.
