摘要
目的:建立快速、准确、灵敏地测定D-木糖含量的高效液相色谱方法。方法:采用氨基柱分离,乙腈-水(73:27,VV为流动相,流速0.8mL/min,柱温30℃;NQAD漂移管温度50℃,载气为氮气,压力30psi。结果:D-木糖的线性范围为10～120μg/mL(R^2=0.9998),最低检出限为0.7μg/mL,平均加标回收率为101.97%,相对标准偏差(RSD)为0.88%。结论:该方法灵敏、准确、重复性好,适合于D-木糖的检测。
A fast, sensitive, accurate HPLC method with nano quantity analyte detector （NQAD） for the determination of D-xylose was developed. The chromatographic separation was conducted on an amino column using a mobile phase comprising acetonitrile and water （73：27, 11/11） at a flow rate of 0.8 mL/min. The NQAD drift tube temperature was set at 50 ℃ and the N2 pressure at 30 psi. The linear range of D-xylose was 10-120μg/mL （R2 = 0.9998）. The limit of detection was 0.7 μg/mL. The average spike recovery of the developed method was 101.97%, with a relative standard deviation （RSD） of 0.88%. This method is sensitive, accurate, repeatable, and thus suitable for determining D-xylose.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第2期179-181,共3页
Food Science
基金
国家质检总局公益性项目(201310292)
关键词
高效液相色谱
核粒子计数检测器
D-木糖
high-performance liquid chromatography
nano quantity analyte detector
D-xylose
作者简介
蔡秀娟（1985-），女，硕士研究生，研究方向为营养与食品安全。E—mail：caixiujuan85@qq．com
通信作者：许建中（1954-），男，教授级高工，本科，研究方向为生物化工。E—mail：hyss．xjz@163．com
