摘要
目的:探索丹参饮口服液的质量标准鉴定方法。方法:采用薄层色谱法鉴别丹参,紫外-可见分光光度法和高效液相色谱法鉴别并测定丹参饮口服液中丹参素钠的含量。色谱条件:Inertsil ODS-SP C18柱(250mm×4.6mm,5μm),柱温40℃,流动相甲醇-1%冰乙酸(13∶87),二元梯度洗脱,流速1.0mL.min-1,检测波长280nm。结果:丹参饮口服液以及丹参药材在薄层色谱中可获得清晰的斑点。丹参素钠紫外-可见光谱的最大吸收峰在282nm。丹参素钠在0.83～6.64μg线性关系良好,r=0.999 5;加样回收率试验(n=6)测得平均回收率为97.22%,RSD为1.17%。结论:薄层色谱法鉴别丹参,紫外-可见光谱、HPLC测定丹参饮口服液中丹参素钠的含量,专属性强、重现性好,简便可靠。
Objective: To explore a quality standard identification method for Salvia Oral Liquid.Methods: A thin-layer chromatography was used to identify salvia,and UV-visible spectrophotometry and HPLC were used to determine Sodium Danshensu content in Salvia Oral Liquid.Chromatographic conditions: Inertsil ODS-SP C18 column was 250 mm×4.6 mm,5 μm,with 40 ℃ column temperature,mobile phase methanol and 1% acetic acid(13:87),binary gradient elution with a flow rate of 1.0 mL·min-1,detection wavelength at 280 nm.Results: Salvia Oral Liquid and salvia herbs had clear spots in the TLC.The maximum absorption peak of Sodium Danshensu UV-visible spectra was at 282 nm,which had a good linear relationship at the range of 0.83 ~ 6.64 μg,r= 0.999 5,97.22% of average recovery rate(n = 6),RSD was 1.17%.Conclusion: TLC identification was for Salvia,and UV-visible spectroscopy and HPLC determination for Sodium Danshensu content in Salvia Oral Liquid.The method is exclusive,simple and reliable with a good reproducibility.
出处
《云南中医中药杂志》
2013年第1期61-63,89,共3页
Yunnan Journal of Traditional Chinese Medicine and Materia Medica
基金
泸州市科技局课题(LSCQ<2010>41号)资助
作者简介
蒲清荣，本科，副主任中药师。从事中药制剂研究．Tel：0830-3162253．E-mail：365369747@qq．com
