摘要
为建立一种快速检测牛病毒性腹泻病毒病原的方法,本研究根据GenBank上登录的牛病毒性腹泻病毒(bovine vi-ral diarrhea virus,BVDV)基因组序列,设计1对引物,建立了检测BVDV的一步法RT-PCR方法。该方法对牛传染性鼻气管炎病毒、猪瘟病毒、牛副流感病毒3型的扩增结果均为阴性,检测的敏感性达1ng RNA。该一步法RT-PCR方法具有良好的特异性、敏感性、重复性,可以准确快速检测出极低含量的BVDV,将为BVDV的病原检测及分子流行病学调查等提供一种快速、灵敏、特异、准确的分子生物学检测方法。
To establish a rapid bovine viral diarrhea virus（BVDV）pathogen detection methods,based on BVDV gene sequence in GenBank,we synthesized one pair of primers,established one step RT-PCR for BVDV detection.The method was used for infectious bovine rhinotracheitis virus（IBRV）,classical swine fever virus（CSFV）,bovine para influenza virus 3（BPIV3）,all of the PCR results were negative,the sensitivity was 1ng RNA.The established one step PCR method had good specificity,sensitivity,reproducibility,it could detect very low levels of BVDV quickly and accurately,it provided a kind of rapid,sensitive,specific and precise molecular biology detection method for pathogen detection and molecular epidemiology of material such as BVDV.
出处
《中国畜牧兽医》
CAS
北大核心
2013年第1期66-69,共4页
China Animal Husbandry & Veterinary Medicine
作者简介
作者简介：王春庆（1972-），男，青海人，专科，研究方向：动物疾病。
