摘要
The haemocompatibility of Ti-3Zr-2Sn-3Mo-25Nb biomedical alloy was studied after surface heparinization. A layer of sol-gel TiO2 films was applied on the alloy samples followed by active treatment in the bio-functionalized solution for introducing the OH- and groups, and then the heparin was immobilized on the active TiO2 films through the electrostatic self assembly technology. It is shown that the heparinized films are mainly composed of anatase and rutile with smooth and dense surface. In vitro blood compatibility was evaluated by haemolysis test, clotting time and platelet adhesion behavior tests. The results show that the haemocompatibility of the alloy could be significantly improved by surface heparinization.
为了提高新型近β钛合金Ti-3Zr-2Sn-3Mo-25Nb(TLM)的抗凝血性能,先通过优化溶胶凝胶技术在TLM合金试样表面制备出一层致密、均匀的TiO2薄膜,再结合表面官能化处理和静电层自组装技术,在TiO2薄膜表面构建一个多层肝素功能涂层。通过XRD分析改性处理后TLM合金表面涂层的相组成,借助SEM、AFM研究功能涂层的表面形貌和微观特征,通过动态凝血时间、溶血率、血小板粘附实验对TLM合金表面改性前后的抗凝血性能和血液相容性进行评估。结果表明,经表面肝素化修饰处理后,TLM合金试样表面光滑、平整,同时材料表面的动态凝血时间延长,溶血率明显降低,试样表面粘附的血小板很少且没有被明显激活,TLM合金的抗凝血性能和血液相容性均得到了明显的改善。
基金
Project (31100693/C100302) supported by the National Natural Science Foundation of China
Project (31011120049) supported by the Australia-China Special Fund, International Science Linkages Program co-supported by the Department of Innovation, Industry, Science and Research of Australia, and the Ministry of Science and Technology and National Science Foundation of China
Project(2010ZDKG-96) supported by the Major Subject of "13115" Programs of Shaan’xi Province, China
Project (2012CB619102) supported by the National Basic Research Program of China
