摘要
由加油站附近污染土壤分离到一株好氧共代谢降解三氯乙烯的菌株Em-1,对其进行了形态学、生理生化和分子鉴定,表明为假单胞菌属。该菌可以利用甲苯为唯一碳源生长,可以以甲苯为生长底物降解三氯乙烯。对Em-1在甲苯中的生长曲线进行了分析,发现经过活化的菌株在甲苯培养基中8 h左右进入对数生长期。在摇瓶水平上进行了连续降解实验,表明甲苯经过24 h即消耗完毕,在含200 mg/L甲苯和50 mg/L三氯乙烯的无机盐培养基中,经168 h培养,三氯乙烯去除率达29.6%。为研究微生物共代谢降解三氯乙烯提供了借鉴。
A novel cometabolic trichloroethylene（TCE） degrading strain Em-1 was isolated from the soil nearby a gas station and characterized morphologically,biochemically and physiologically.Combined with 16S rRNA sequence analysis,it was identified as Pseudomonas sp.It could grow with toluene as the sole carbon source.Analysis of growth curve showed that Em-1 could reach the logarithmic phase around 8 h.Continuous sampling showed that toluene was exhausted within 1 d.With 168 h incubation in mineral salt media with 200 mg/L toluene and 50 mg/L TCE,the TCE bioremoval efficiency could reach 29.6%.The study would provide some reference for further study in TCE biodegradation by co-metabolism.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2013年第1期61-64,119,共5页
Environmental Science & Technology
基金
国家自然科学基金项目(10905035
41101235)
江苏省滩涂生物资源与环境保护重点建设实验室开放课题资助
关键词
好氧共代谢
生物降解
假单胞菌属
三氯乙烯
甲苯
aerobic cometabolism
biodegradation
Pseudomonas sp.
trichloroethylene
toluene
作者简介
张祥胜（1976-），男，副教授，博士，主要从事环境微生物和离子束生物学效应的研究，（电话）0515-88233189（电子信箱）yctu_shengwu@163．com
