摘要
目的用数字化基因表达谱分析大鼠放射性肺损伤发生过程中的差异表达基因,寻找放射性肺损伤发生的关键基因。方法将20只Wistar大鼠随机分为单纯照射组(模型组)10只、空白对照组(对照组)10只。分次采用6MV-X直线加速器照射模型组大鼠右肺,对照组不予照射。于首次照射模型大鼠开始计时,两组均于第6、12周末随机活杀5只大鼠,取其右肺,提取其肺组织总mRNA,经过处理后进行数字化基因表达谱分析,对比两组基因差异表达情况。结果第6周末模型组对比对照组的差异基因有1050个,上调基因为669个,下调基因为381个。第12周末模型组对比对照组的差异表达基因有2050个,上调基因为833个,下调基因为1271个。其中MMP2、MMP9、MMP12、MMP14、TIMP1在第6、12周末模型组中均有高表达,且各基因在第12周末时差异表达更明显,其中MMP12差异表达最为显著。而第12周末模型组对比对照组,胶原蛋白、层黏连蛋白及纤维素连接蛋白等纤维化相关基因明显高表达。结论大鼠放射性肺损伤的不同时期差异表达基因不同,MMP2、MMP9、MMP12、MMP14、TIMP1参与了放射性肺损伤的形成,MMP14为放射性肺损伤的又一敏感基因,MMP12可能是放射性肺损伤发生的关键基因。
Objective To analyze the differential expression in the process of rats with radioactive pulmonary in- jury by digital gene expression profile in order to find the key point genes. Methods 20 Wistar rats were randomly divid- ed into the irradiation group （ medel group, n = 10） and blank control group （ control group, n = 10）. The right lung of model rats were fractionated irradiated, by 6MV-X licar accelerator while rats in control group was not irradiated. 5 rats in both groups were randomly killed at 6th , 12th weekend after the first radiation time. The right lung was taken our, and the total mRNA of lung tissue was extracted. Digital gene expression profiling was performed after handling, and then dif- ferential expressions of the two groups were compared. Results There were 1050 differentially expressed genes between model and control group, 669 up-regulated genes and 381 down-regulated genes at the 6th weekend. There were 2050 dif- ferentially expressed genes between model and control group, 833 up-regulated genes and 1271 down-regulated genes at the 12th weekend. MMP2, MMP9, MMP12, MMP14 and TIMP1 had obvious high expressions in model group at both 6th and 12th weekend, and the discrepancy were more apparently at 12th weekend. The MMP12 was the most significant one. At the 12th weekends the collagen protein, layer synechia protein and cellulose connected protein expressions were markedly higher in model group than those of control group. Conclusion Different periods have differentially expressed genes in rats＇ radioactive lung injury, MMP2, MMP9, MMP12, MMPI4 and TIMP1 are involved in the formation of the radioactive lung injury, MMP14 is another sensitive gene of radioactive lung injury, and MMPI2 may be the key gene of radioactive lung injury.
出处
《解放军医药杂志》
CAS
2013年第1期14-17,共4页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
基金
全军"十二五"中医药重点项目(10ZYZ105)
关键词
数字化基因表达谱
放射疗法
放射性肺损伤
基因表达
差异基因
大鼠
Wistar
Digital gene expression profii
Radiotherapy
Radioactive lung injury
Gene expression
Differenti- ally expressed gene
Rat, Wistar
作者简介
[通讯作者]王炳胜，E-mail：bingshengw@126．com
