摘要
采用十八烷基硅烷键合硅胶柱Welchrom-C18(4.6mm×250mm,5μm)为色谱柱,柱温30℃,流动相为乙腈-甲醇-5mL/L醋酸溶液(体积比为16∶26∶58),流速为1.00mL/min,检测波长287nm,进样量10μL。结果表明,水飞蓟宾的进样量在0.219μg～5.475μg(r=0.999 999,n=6)范围内与其峰面积积分值呈良好的线性关系,平均加样回收率为101.211%(n=6)。表明建立的高效液相色谱法快速、准确,重现性和精密度均良好,可为中草药复方"健肝散"的质量控制和评价提供技术支撑。
To establish a HPLC method for determinatioti of silybin ih ＂jiangahgan＂ Of veterinary drug, the content determination was performed on Welchrom-C18（4.6 mm × 250 mm , 5 μm） ; with the mobile phase consisted of acetonitrile-methanol-5 mL/L acetic acid （16 ： 26 ： 58）. The fii3w rate was 1.0 mL/min,and the detection wavelength of silybin was set at 287 tim, The column temperature was maintained at 30 ℃ and injection volume was 10 /LL, In result,the linea; range Of silybin was from 0. 219 to 5. 475 μg（r- 0. 999 999） ,and the average recovery were 101, 2il N, Iti conclusion, the method was simple,accurate, re- producible and precise ,which would be suitable for content determination,quality control and evaluation of silybin in ＂Jiangansan＂.
出处
《动物医学进展》
CSCD
北大核心
2013年第1期43-46,共4页
Progress In Veterinary Medicine
基金
四川省科技支撑计划项目(2008S20131)
作者简介
张盈娇（1981-），女，四川资中人，硕士，助理研究员，主要从事中兽药药效与残留研究。
通讯作者
