摘要
目的通过对菝葜各化学部位的药效学筛选,确定其治疗慢性盆腔炎疾病(CPID)的有效部位,为菝葜制剂的二次开发提供科学依据。方法采用苯酚胶浆注入大鼠子宫造成大鼠CPID模型,观察菝葜各化学部位对CPID模型大鼠的治疗作用;采用二甲苯致小鼠耳肿胀法和大鼠棉球肉芽肿法,探讨菝葜各化学部位的抗炎作用。结果菝葜乙酸乙酯部位的高剂量组能显著降低CPID模型大鼠的子宫炎症反应,对小鼠耳肿胀和大鼠棉球肉芽肿的抗炎作用与模型对照组比较均有显著性差异(P<0.01);中剂量组也能明显改善炎症程度。总体抗炎作用优于总提液组。正丁醇部位作用不明显。结论菝葜的乙酸乙酯部位为菝葜抗炎及抗慢性盆腔炎的有效部位。
Objective To determine the effective fraction of Smilax for treatment of chronic pelvic inflammatory disease （CPID） by pharmacodynamic screening as the basis for further development of sarsaparilla preparations. Methods The chemical fractions of Smilax were administered intragastrically in rat models of CPID induced by injecting phenol mucilage into the uterus to observe the therapeutic effects. The anti-inflammatory effects of different extract fractions from Smilax were tested in mice with xylene-induced ear edema and in rats with cotton-ball-induced granuloma. Results High-dose ethyl acetate extract of Smilax could obviously inhibit uterus inflammation in rats with CPID, showing also strong anti-inflammatory effects against ear edema in mice and granuloma in rats （P〈0.01）. The moderate dose of ethyl acetate extract also obviously ameliorated the inflammation. Both the ethyl acetate extract fraction and the total extract fraction of Smilax showed anti-inflammatory effects, while the former produced strong effects while the latter has only weak actions. Conclusion The ethyl acetate extract fraction of Smilax is the effective fraction to produce anti-inflammatory and anti-CPID effects.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2013年第1期145-149,共5页
Journal of Southern Medical University
基金
广东省建设现代化产业体系技术创新滚动项目计划(20101022106)
广东省科技计划项目(2009B050500003)
关键词
菝葜
有效部位
慢性盆腔炎
抗炎
筛选
Smilax
effective fractions
chronic pelvic inflammation
anti-inflammation
screening
作者简介
马云，在读博士研究生，主管药师，E—mail：mayun329@126.com
通信作者：侯连兵，博士生导师，主任药师，教授，E-mail：hlianbing@163.com
