摘要
金刚石是一种由纯碳组成的矿物,属原子晶体,是自然界中最坚硬的物质。粒径在1～100 nm的金刚石称为纳米金刚石,Greiner等[1 3]用爆炸的方法合成并确定了其物理结构及化学键连接方式,同时纳米金刚石也是碳纳米家族中一个重要的成员。
The purpose of this study is to investigate the intracellular transporters effect and the cytotoxicity of carboxyl nanodiamond（CND） podophyllotoxin（PPT）.Nanodiamond（ND） was treated with mixed carboxylic acid and finally got 64 nm CND by centrifugation,and then it was reacted with PPT to form CNDPPT.UV spectrophotometry was used to calculate the content of PPT in CND-PPT,the particle size distribution and zeta potential were measured by Dynamic laser scattering instrument.CND,PPT,CND-PPT and CND ＋ PPT（physical mixture of CND and PPT） were characterized by Fourier transform infrared spectroscopy,at the same time,thermal analysis and element analysis were used to estimate the content of the PPT in CND-PPT.The affect of CND,PPT,CND-PPT on HeLa cell was measured with MTT assay.The results showed that content of PPT combined with CND accounted for about 10%.MTT assay showed that CND has low cytotoxicity and CND-PPT can increase the water soluble of PPT.As a conclusion,CND as a hydrophilic pharmaceutical carrier combined with PPT is able to increase the water solubility of PPT,at low concentration,CND-PPT can enhance the antitumor activity in comparison with PPT,so CND can be used as a potential anticancer drug carrier.
出处
《药学学报》
CAS
CSCD
北大核心
2013年第1期149-154,共6页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目(81273440)
甘肃省中医药重点项目(GZK-2009-1)
关键词
纳米金刚石
鬼臼毒素
转运载体
nanodiamond
podophyllotoxin
intracellular transporter
作者简介
通讯作者Tel：86-931-8915683，Fax：86—931-8911255，E-mail：nijm@lzu．edu．cn
