摘要
[目的]为提高纤维素酶产生菌的酶活力。[方法]以抚顺近郊堆积腐烂秸秆的土壤为分离源,利用CMC刚果红平板培养基筛选出纤维素水解圈与菌落直径比值(Hc值)较大的菌株Y-07。以Y-07为出发菌株,在最适诱变剂量条件下,对其进行紫外线诱变和亚硝酸诱变。[结果]经过3轮复筛,紫外线诱变后的U10菌株为试验中获得的高产纤维素酶菌株,其酶活最高达到2.499 IU/ml,其活性是Y-07的3.41倍。[结论]该方法为进一步提高纤维素酶产生菌的酶活力奠定了基础。
[ Objective] The research aimed to improve enzyme activity of cellulase production strain. [ Method] A strain named Y-07 was iso- lated from rotten straw soil^nearby the suburb of Fushun with culture medium containing the carboxymethylcellulose sodium and Congo Red in accordance with its larger size of ratio of hydrolysis ring to colony diameter. With the strain of Y-07 as starting strain, the strain of Y-07 was mutagenized by ultraviolet radiation and nitrous acid mutagenesis under the most appropriate amount of mutation. [ Result] Mter three rounds of secondary screening, the strain of U10 was achieved with the biggest cellulase activity 2.5 IU/ml which was 3.4 times of Y-07 strain. [ Conclusion ] The method provided the basis for further improvements of enzyme activity of cellulase production strain.
出处
《安徽农业科学》
CAS
2012年第34期16510-16512,共3页
Journal of Anhui Agricultural Sciences
关键词
纤维素酶
紫外诱变
亚硝酸诱变
酶活
Celluhse
UV radiation mutagenesis
Nitrite mutagenesis
Enzyme activity
作者简介
王美珠(1990-),女,辽宁抚顺人,本科生,专业:生物化工;通讯作者:曹月坤,副教授,从事生物工程方面的研究。E-mail:cyk2611765@163.com。
