摘要
采用常规病理组织学、Howell红氨酸染色、原子吸收光谱检测等方法,对硫酸铜处理28 d的剑尾鱼进行组织病理学变化、Cu2+在组织中沉积和蓄积研究。硫酸铜中毒的主要病理变化为:鳃小片粘液分泌增多,鳃丝上皮细胞肿胀、变性、坏死、增生,鳃小片融合,鳃丝棒状化;肝脏水肿,肝静脉和肝窦淤血、溶血,肝细胞的水泡变性,弥漫性坏死、核固缩、核碎裂、核溶解,胰腺细胞的酶原颗粒减少;Cu2+主要沉积和蓄积在肝脏中。研究结果表明,肝脏病理变化具有代谢性中毒特征,并与肝脏中Cu2+蓄积量具有一致性;肝脏是Cu2+的重要蓄积器官,蓄积量与时间、浓度具有相关性;Howell红氨酸染色后Cu2+在肝脏中的显示和病理变化可作为铜中毒的重要指标。
In order to study the toxic effects of copper sulfate on swordtail fish, toxicity tests were carried out. The methods of histopathology, Howell＇s rubeanic acid staining and atomic absorption spectrum meter were used. A comprehensive scientific evaluation was made on histopathologieal changes and the accumulation and deposition of copper sulfate in the swordtail fish. The results of histopathology showed that damage of the gills included epithelial cell changed such as rupture, peeling of lamellar epithelium, lamellar fusion, degeneration, necrosis, hyperplasia, cellular hypertrophy, and elaviform. Damage to livers included edema, necrosis, hepatoeellular vacuolar degeneration, extensive necrosis, karyopyknosis, karyorrhexis, karyolysis, congestion and hyperemia. The result of rubeanic acid staining and the atomic absorption spectrum meter showed that copper ions were mainly deposited and accumulated in livers. Pathological changes in livers have relativity with accumulated quantity; The livers are mainly accumulating organ and accumulated quantity have relativity with duration and concentration; Copper ion deposit and pathological changes in livers are the important index.
出处
《广东农业科学》
CAS
CSCD
北大核心
2012年第21期136-138,F0003,共4页
Guangdong Agricultural Sciences
基金
科技部科研院所社会公益研究专项(2004DIB1J029)
国家现代农业产业技术体系建设专项(nycytx-49-14)
关键词
剑尾鱼
硫酸铜
中毒
组织蓄积
病理学
swordtail fish
copper sulfate
intoxication
tissue accumulation
pathology
作者简介
作者简介：常藕琴（1972-），女，硕士，副研究员，E—mail：changouqin@163．com
通讯作者：吴淑勤（1956），女，研究员，E—marl：sqwxm@163．net
