摘要
研究副干酪乳杆菌M5-L抑制宋内志贺氏菌(S.sonnei)对HT-29细胞黏附的作用及其影响抑制黏附作用的表面分子分析。结果表明M5-L能够显著的抑制S.sonnei的黏附作用,在排除、竞争和取代黏附实验中,抑制率分别为55%、33%和30%,差异显著(p<0.05),高碘酸钠处理后,M5-L的抑制黏附作用没有变化,表明碳水化合物并没有参与到黏附抑制过程中。而经过5mol/L LiCl处理后,M5-L的抑制黏附作用下降,说明S-层蛋白参与了M5-L抑制S.sonnei对HT-29细胞的黏附作用。此外,M5-L的S-层蛋白能够显著抑制S.sonnei的黏附作用。
L.paracasei subp. paracasei M5-L inhibition of Shigella sonnei adhesion to HT-29 cells and the type of molecules in the M5-L cells surface which effecting the inhibition of S.sonnei were investigated. In exclusion, competition and displacement assays, L.paracasei subp. paracasei M5-L exhibited significant inhibitory activity （p〈0.05）,preventing 55% ,33% and 30% of the S.sonnei cells from adhering to HT-29 cell respectively. Treatment with metaperiodate on the M5-L did not affect the inhibitory activity,which showed that the carbohydrates did not involved in the inhibition adherence. When the M5-L treated with 5mol/L LiCI,the inhibition activity was decreased significantly,which indicated the S-layer protein was the important molecules in the inhibition activity. In addition,the S-layer proteins exhibited strongly inhibitory effects on the S.sonnei adherence to HT-29 ceils.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第24期99-102,共4页
Science and Technology of Food Industry
基金
中央高校基本科研业务费专项资助项目(HIT.NSRIF.2012082)
中国博士后科学基金资助项目(2012M510093)
作者简介
作者简介：张英春（1975-），女，博士，讲师，研究方向：乳酸菌及其活性产物研究。
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