期刊文献+

草莓抗白粉病遗传图谱及其SSR标记初分析 被引量:10

Genetic Mapping and Preliminary Analysis of SSR Marker for Powdery Mildew Resistance in Strawberry
在线阅读 下载PDF
导出
摘要 以易感草莓白粉病品种达赛莱克特和高抗品种甜查理杂交的220个杂种F2代群体材料为作图群体,利用146对SSR标记构建了包含两个连锁群和7个SSR标记的分子遗传连锁图谱,平均遗传图距8.85 cM。通过对抗草莓白粉病相关的SSR标记进行初步遗传分析,结果 FSS50和FSS121与抗性相关基因连锁比较紧密,遗传距离分别为1.3 cM和3.3 cM,理论上可应用于白粉病抗性种质的辅助选择育种实践中。 Highly - resistant and highly - susceptible strawberry cultivars and their hybrid progenies were evaluated by phenotype observation in the field and in greenhouse. One hundred and forty - six pairs of SSR ( Simple sequence repeat) markers were tested to construct a molecular genetic linkage map containing two linkage groups and seven SSR markers based on a strawberry population con- sisting of 220 F2 individuals from the hybridization between Darselect (susceptible) and Sweet Chadie (resistant), covering the two linkage group with an average interval length of 8.85 cM. FSSS0 and FSS121 were closely linked with resistant genes for strawberry powdery mildew based on the preliminary genetic analysis of SSR markers, with 1.3 cM and 3.3 cM genetic distance, respectively. They could be applied to the practice of powdery mildew resistant germplasm assisted selection breeding in theory.
出处 《江西农业学报》 CAS 2012年第11期49-52,共4页 Acta Agriculturae Jiangxi
基金 上海市自然科学基金项目(10ZR1426700) 上海市农委重大项目(沪农科重字(2010)第4-1号) 农业部公益性行业(农业)科研专项(No.201003064-5)资助
关键词 草莓 白粉病抗性 SSR标记 遗传图谱 Strawberry Powdery mildew resistance SSR marker Genetic mapping
作者简介 刘建成(1983-,男,硕士,研究方向:病源微生物相关分子生物学研究。 通讯作者:高清华。
  • 相关文献

参考文献11

  • 1Davis T M ,Yu H. A linkage map of the diploid strawberry, Fragaria vesea [ J ]. Journal of Heredity, 1997,88 ( 3 ) :215 - 221.
  • 2Sargent D J,Davis T M,Tobutt KR,et al. A genetic linkage map of microsatellite, gene -specific and morphological markers in diploid Fragaria [ J ]. Theor Appt Genet., 2004,109 ( 7 ) : 1385 - 1391.
  • 3Sargent D J, Clarke J, Simpson D W, et al. An enhanced microsat- ellite map of diploid Fragaria [ J ]. Theor Appl Genet. ,2006,112 (7) :1349 - 1359.
  • 4Spigler R B,Lewers K S,Main D S,et al. Genetic mapping of sex determination in a wihl strawberry, Fragaria virginiana, reveals earliest form of sex chromosome [ J ]. Heredity ,2008 : 1 - 11.
  • 5Mathieu R G,Estelle L K, Laure B, et al. Comparative Genetic Mapping Between Octaploid and Diploid Fragaria Species Reveals a High Level of Colinearity Between Their Genomes and the Essentially Disomic Behavior of the Cuhivated Octoploid Strawberry [J]. Geneties,2008,179:2045 - 2060.
  • 6Lerceteau - Kohler E,Guerin G,Laigret F,et al. Characterization of mixed disomic and polysomic inheritance in the oetoploid strawberry ( Fragaria x ananassa ) using AFLP mapping[J].Theor. Appl. Genet. ,2003,107:619 -628.
  • 7James C M,Lesemann S S,Down G J. Modified AFLP Analysis Method for Species With Small Genomes [ J ]. Plant Molecular Biology Reporter,2003,21:303 - 307.
  • 8Hadonou A M,Sargent D J,Wilson F,et al. Development of microsatellite markers in Fragaria, their use in genetic diversity analysis and their potential for genetic linkage mapping [ J ]. Genome ,2(104,47:429 - 438.
  • 9Sargent D J, Hadonou A M,Simpson D W. Development and char acterization of polymorphic micro - satellite markers from Fragar in viridis, a wild diploid strawberry [J]. Mol Ecol Notes,2003 3:550 - 552.
  • 10Sargent D J,Rys A,Nier S,et al. The development and mapping of functional markers in Fragaria and their transferability and potential for mapping in other genera [J].Theor Appl Genet, , 2007,114 (2) :373 - 384.

同被引文献120

引证文献10

二级引证文献119

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部