摘要
目的用基因分型技术对中国汉族、蒙古族健康人CYP3A4、CYP2C9、CYP2C19、CYP2D6进行基因多态性分析,并对汉族人和蒙古族人基因表型和基因频率进行比较。方法聚合酶链反应-限制性片段长度多态性(PCR-RFLP)对CYP3A4、CYP2C9、CYP2C19、CYP2D6进行分型。结果汉族、蒙古族健康人CYP3A4*5等位基因频率为0,CYP3A4*18等位基因频率分别为0.183 8、0.202 5,CYP2C9*2等位基因频率分别为0.011 0、0.025 3,CYP2C9*13等位基因频率分别为0、0.00 32,CYP2C19*2等位基因频率分别为0.386 0、0.4146,CYP2C19*3等位基因频率分别为0.051 5、0.044 3,CYP2D6*10等位基因频率分别为0.573 5、0.465 2。结论汉族、蒙古族健康人群CYP3A4*18、CYP2C19*2、CYP2C19*3、CYP2D6*10等位基因频率均没有显著性差异;本试验在汉族、蒙古族健康人中未发现CYP3A4*5等位基因;仅在蒙古族健康受试人群中发现1人为CYP2C9*1/*13基因型;蒙古族CYP2C9*2等位基因频率远小于汉族(P=0.023)。
OBJECTIVE To study the genotype distribution of cytochrome CYP3A4, CYP2C9, CYP2C19, CYP2D6 in Han and Mongolian Chinese population and the allele and genotype frequency are compared between the Han and Mongolian. METHODS CYP3A4, CYP2C9, CYP2C19, CYP2D6 of Han and Mongolian Chinese population were genotyped by PCR-RFLP. RESULTS The allele frequencies of CYP3A4 ＊ 5 in Han and Mongolian are 0, the allele frequencies of CYP3A4 ＊ 18 in Han and Mongolian are 0. 183 8, 0. 202 5. The allele frequencies of CYP2C9 ＊ 2 in Han and Mongolian are 0. 011 0, 0. 025 3; the allele frequencies of CYP2C9 ＊ 13 in Han and Mongolian are 0, 0. 003 2. The allele frequencies of CYP2C19 ＊ 2 in Han and Mongolian are 0. 386 0, 0. 414 6, the allele frequencies of CYP2C19 ＊ 3 are 0. 051 5, 0. 044 3. The allele frequencies of CYP2D6 ＊ 10 in Han and Mongolian are 0. 573 5, 0. 465 2. CONCLUSION The study shows that there are no significant ethnic differences in the distribution of CYP3A4 ＊ 18, CYP2C19 ＊ 2, CYP2C19 ＊ 3, CYP2D6 ＊ 10 genotypes in Han and Mongolian. The CYP3A4 ＊ 5 genotype is not found in this study and only one CYP2C9 ＊ 1/＊ 13 genotype is found in Mongolian. For CYP2C9 ＊ 2, the Mongolian is significantly lower than the Han, （P =0. 023）.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2012年第24期2017-2022,共6页
Chinese Pharmaceutical Journal
作者简介
作者简介：夏东亚，男，主任药师，硕士生导师研究方向：药代动力学，临床药学
通讯作者：郭涛，男，主任药师，博士生导师研究方向：药物制剂研究，临床药学Tel：（024）28897067E-mail：syzygcp@163．com
